SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 13G

INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT

TO RULES 13d-1(b), (c), AND (d) AND AMENDMENTS THERETO FILED

PURSUANT TO RULE 13d-2(b)

Amendment No. 2

Applied Neurosolutions Inc.

(Name of Issuer)

Common Stock

(Title of Class of Securities)

03823Y-203

(CUSIP Number)

December 31, 2010

(Date of Event Which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

þ Rule 13d-1(b)

o Rule 13d-1(c)

o Rule 13d-1(d)

______________

* The remainder of this cover page shall be filled out for a reporting person's initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter the disclosures provided in a prior cover page.

The information required in the remainder of this cover page shall not be deemed to be "filed" for the purpose of Section 18 of the Securities Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes).

CUSIP No. 03823Y-203

1.	NAME OF REPORTING PERSONS. I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (ENTITIES ONLY) Wilmington Trust Corporation
2.	CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP* (a) þ (b) o
3.	SEC USE ONLY
4.	CITIZENSHIP OR PLACE OF ORGANIZATION Delaware corporation
Number of Shares Beneficially Owned by Each Reporting Person With:		5.	SOLE VOTING POWER 269,738
		6.	SHARED VOTING POWER 0
		7.	SOLE DISPOSITIVE POWER 269,738
		8.	SHARED DISPOSITIVE POWER 0
9.	AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 269,738
10.	CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES* o N/A
11.	PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 6.1%
12.	Type of Reporting Person * HC

CUSIP No. 03823Y-203

1.	NAME OF REPORTING PERSONS. I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (ENTITIES ONLY) Wilmington Trust Company, in various fiduciary capacities
2.	CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP* (a) þ (b) o
3.	SEC USE ONLY
4.	CITIZENSHIP OR PLACE OF ORGANIZATION Delaware banking corporation
Number of Shares Beneficially Owned by Each Reporting Person With:		5.	SOLE VOTING POWER 269,738
		6.	SHARED VOTING POWER 0
		7.	SOLE DISPOSITIVE POWER 264,021
		8.	SHARED DISPOSITIVE POWER 0
9.	AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 269,738
10.	CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES* o N/A
11.	PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 6.1%
12.	Type of Reporting Person * BK

CUSIP No. 03823Y-203

1.	NAME OF REPORTING PERSONS. I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (ENTITIES ONLY) Wilmington Trust FSB, in various fiduciary capacities
2.	CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP* (a) þ (b) o
3.	SEC USE ONLY
4.	CITIZENSHIP OR PLACE OF ORGANIZATION Delaware banking corporation
Number of Shares Beneficially Owned by Each Reporting Person With:		5.	SOLE VOTING POWER 6,150
		6.	SHARED VOTING POWER 0
		7.	SOLE DISPOSITIVE POWER 0
		8.	SHARED DISPOSITIVE POWER 0
9.	AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 6,150
10.	CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES* o N/A
11.	PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 0.0%
12.	Type of Reporting Person * BK

CUSIP No. 03823Y-203

ITEM 1(A). NAME OF ISSUER:

Applied Neurosolutions, Inc.

ITEM 1(B). ADDRESS OF ISSUER'S PRINCIPAL EXECUTIVE OFFICES:

50 Lakeview Pkwy, Suite 111, Vernon Hills, IL 60061

ITEM 2(A). NAME OF PERSON FILING:

Wilmington Trust Corporation and Wilmington Trust Company

ITEM 2(B). ADDRESS OF PRINCIPAL BUSINESS OFFICE, OR IF NONE, RESIDENCE:

1100 North Market Street

Wilmington, DE 19890

ITEM 2(C). CITIZENSHIP:

Wilmington Trust Corporation is a Delaware corporation;

Wilmington Trust Company is a Delaware banking corporation.

ITEM 2(D). TITLE OF CLASS OF SECURITIES:

Common Stock

ITEM 2(E). CUSIP NUMBER:

03823Y-203

____________________________________________________________________

ITEM 3. IF THIS STATEMENT IS FILED PURSUANT TO RULE 13D-1(B), OR 13D-2(B) OR (C), CHECK WHETHER THE PERSON FILING IS A:

(a) o Broker or dealer registered under Section 15 of the Exchange Act.

(b) þ Bank as defined in Section 3(a)(6) of the Exchange Act.

Wilmington Trust Company is a Bank and is a direct, wholly- owned subsidiaries of Wilmington Trust Corporation.

CUSIP No. 03823Y-203

(d) o Investment company registered under Section 8 of the Investment Company Act.

(e) o An investment adviser in accordance with Rule 13d-1(b)(1)(ii)(E);

(f) oAn employee benefit plan or endowment fund in accordance with Rule 13d-1(b)(1)(ii)(F);

(g) þA parent holding company or control person in accordance with Rule 13d-1(b)(1)(ii)(G);

Wilmington Trust Corporation is a Parent Holding Company.

(h) oA savings association as defined in Section 3(b) of the Federal Deposit Insurance Act;

(i) oA church plan that is excluded from the definition of an investment company under Section 3(c)(14) of the Investment Company Act;

(j) þ Group, in accordance with Rule 13d-1(b)(1)(ii)(J).

Wilmington Trust Corporation, Wilmington Trust Company and Wilmington Trust FSB.

ITEM 4. OWNERSHIP.

Provide the following information regarding the aggregate number and percentage of the class of securities of the issuer identified in Item 1.

(a) Amount beneficially owned: 269,738

(b) Percent of class: 6.1%

(i) Sole power to vote or to direct the vote shares 269,738

(ii) Shared power to vote or to direct the vote shares 0

(iii) Sole power to dispose or to direct the disposition of shares 260,421

(iv) Shared power to dispose or to direct the disposition of shares 0

Number of shares as to which Wilmington Trust Company has:

(i) Sole power to vote or to direct the vote shares 263,588

(ii) Shared power to vote or to direct the vote shares 0

(iii) Sole power to dispose or to direct the disposition of shares 260,421

(iv) Shared power to dispose or to direct the disposition of shares 0

ITEM 5. OWNERSHIP OF FIVE PERCENT OR LESS OF A CLASS.

If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than five percent of the class of securities, check the following o

ITEM 6. OWNERSHIP OF MORE THAN FIVE PERCENT ON BEHALF OF ANOTHER PERSON.

Not applicable.

ITEM 7. IDENTIFICATION AND CLASSIFICATION OF THE SUBSIDIARY WHICH ACQUIRED THE SECURITY BEING REPORTED ON BY THE PARENT HOLDING COMPANY.

Wilmington Trust Company: BK

ITEM 8. IDENTIFICATION AND CLASSIFICATION OF MEMBERS OF THE GROUP.

Wilmington Trust Corporation: HC

Wilmington Trust Company: BK

Wilmington Trust FSB BK

ITEM 9. NOTICE OF DISSOLUTION OF GROUP.

Not applicable.

ITEM 10. CERTIFICATIONS.

By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect.

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

	WILMINGTON TRUST CORPORATION WILMINGTON TRUST COMPANY WILMINGTON TRUST FSB

February 14, 2011	By:	/s/ Michael A. DiGregorio
		Michael A. DiGregorio
		Executive Vice President, General Counsel and Secretary

Attention. Intentional misstatements or omissions of fact constitute federal criminal violations (see 18 U.S.C. 1001).

APPLIED NEUROSOLUTIONS INC SC 13G/A