Exhibit 21

APPENDIX A

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CONSOLIDATED (MORE THAN 50% OWNED AND CONTROLLED) SUBSIDIARIES

OF NORFOLK SOUTHERN CORPORATION AND STATES OF INCORPORATION

AS OF JANUARY 31, 2026


	STATE OR COUNTRY OF INCORPORATION
General American Insurance Company		Georgia
Norfolk Southern Properties, Inc.		Virginia
Norfolk Southern Railway Company		Virginia
NS Fiber Optics, Inc.		Virginia
T-Cubed of North America, LLC		Delaware
Thoroughbred Technology and Telecommunications, LLC		Virginia


Norfolk Southern Railway Company Subsidiaries
Airforce Pipeline, Inc.						North Carolina
Alabama Great Southern LLC						Virginia
Alabama Great Southern Railroad Company, The						Alabama
Camp Lejeune Railroad Company						North Carolina
Central of Georgia Railroad Company						Georgia
Chesapeake Western Railway						Virginia
Cincinnati, New Orleans and Texas Pacific Railway LLC, The						Ohio
Citico Realty Company						Virginia
CNOTP LLC						Ohio
Georgia Southern and Florida Railway Company						Georgia
GSFR LLC						Georgia
High Point, Randleman, Asheboro and Southern Railroad Company						North Carolina
HPRASR LLC						North Carolina
Interstate Railroad Company						Virginia
Lamberts Point Barge Company, Inc.						Virginia
Mobile and Birmingham Railroad Company						Alabama
Norfolk and Portsmouth Belt Line Railroad Company						Virginia
Norfolk Southern - Mexico, LLC						Virginia
NorfolkSouthernMexicana, S. de R.L. de C.V.						Mexico
North Carolina Midland Railroad Company, The						North Carolina
NS Spectrum Corporation						Virginia
Rail Investment Company						Delaware
Reading Company, LLC [Virginia]						Virginia
RIC LLC						Delaware
South Western Rail Road Company, The						Georgia
Southern Rail Terminals, Inc.						Georgia
Southern Rail Terminals of North Carolina, Inc.						North Carolina
Southern Region Materials Supply, Inc.						Georgia
State University Railroad Company						North Carolina
Thoroughbred Emissions Research, LLC						Virginia
Thoroughbred Funding, Inc.						Virginia
Triple Crown Services, Inc						Virginia
Virginia and Southwestern Railway Company						Virginia
Wheelersburg Terminal LLC						Virginia

APPENDIX A

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	STATE OR COUNTRY OF INCORPORATION
Norfolk Southern Railway Company Subsidiaries (continued)
Yadkin Railroad Company		North Carolina
Yadkin Railroad Investment LLC		North Carolina


Norfolk Southern Properties, Inc. Subsidiaries
Alexandria-Southern Properties, Inc.						Virginia
Arrowood-Southern Company						North Carolina
Nickel Plate Improvement Company, Inc., The						Indiana
NS Transportation Brokerage Corporation						Virginia
Sandusky Dock Corporation						Virginia
Southern Region Industrial Realty, Inc.						Georgia
Virginia Holding Corporation						Virginia
Westlake Land Management, Inc.						Florida

In addition, NS owns direct or indirect equity interest in:

Central New York Railroad Corporation

Conrail Inc.

Consolidated Rail Corporation and its consolidated subsidiaries

Cooperstown and Charlotte Valley Railway Corporation

CRR Holdings LLC

CRR Investments, Inc.

Delaware Otsego Corporation

DOCP Acquisition, LLC

Green Acquisition Corp.

Indiana Harbor Belt Railroad Company

New York, Susquehanna and Western Railway Corporation, The

Penn Central Communications Company

Susquehanna Properties, Inc.

Susquehanna Bulk Systems, Inc.

Syracuse, Binghamton and New York Railroad Corporation

NORFOLK SOUTHERN CORP 10-K