UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

FORM 10-Q

(Mark One)


☒			QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

For the quarterly period ended June 30, 2025


☐			TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

For the transition period from ___________________ to ___________________

Commission file number 001-41536

Prime Medicine, Inc.

(Exact name of registrant as specified in its charter)


Delaware						84-3097762
(State or other jurisdiction of incorporation or organization)						(I.R.S. Employer Identification No.)

60 First Street, Cambridge, MA						02141
(Address of principal executive offices)						(Zip code)

(617) 465-0013

(Registrant’s telephone number, including area code)

Not applicable

(Former name, former address and former fiscal year, if changed since last report)

Securities registered pursuant to Section 12(b) of the Act:


Title of each class	Trading Symbol(s)	Name of each exchange on which registered
Common stock, par value $0.00001 per share	PRME	Nasdaq Global Market

Indicate by check mark whether the registrant: (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports); and (2) has been subject to such filing requirements for the past 90 days. Yes ☒ No ☐

Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S-T (§232.405 of this chapter) during the preceding 12 months (or for such shorter period that the registrant was required to submit such files). Yes ☒ No ☐

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smaller reporting company. See the definitions of “large accelerated filer,” “accelerated filer” and “smaller reporting company” in Rule 12b-2 of the Exchange Act.


Large accelerated filer	☐	Accelerated filer	☐
Non-accelerated filer	☒	Smaller reporting company	☒
		Emerging growth company	☒

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act.☐

Indicate by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Exchange Act). Yes ☐ No ☒

As of July 31, 2025, the registrant had 134,573,998 shares of common stock, par value $0.00001 per share, outstanding.

SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS

This Quarterly Report on Form 10-Q contains express or implied statements which are made pursuant to the safe harbor provisions of Section 27A of the Securities Act of 1933, as amended, (the “Securities Act”) and Section 21E of the Securities Exchange Act of 1934, as amended (the “Exchange Act”). All statements, other than statements of historical facts, contained in this Quarterly Report on Form 10-Q, including statements regarding our strategy, future operations, future financial position, future revenue, projected costs, prospects, plans, and objectives of management, are forward-looking statements, which are based on management’s belief and assumptions and on information currently available to our management. These statements involve substantial risks, assumptions and uncertainties. The words “anticipate,” “believe,” “envision,” “estimate,” “expect,” “goal,” “intend,” “may,” “plan,” “predict,” “project,” “strategy,” “target,” “potential,” “will,” “would,” “could,” “should,” “continue,” “contemplate,” “vision” and similar expressions are intended to identify forward-looking statements, although not all forward-looking statements contain these identifying words.

The forward-looking statements in this Quarterly Report on Form 10-Q include statements about:

•the initiation, timing, progress and results of our research and development programs, product candidates, preclinical studies and future clinical trials;

•our ability to demonstrate, and the timing of, preclinical proof-of-concept in vivo for multiple programs;

•our ability to advance any current and future product candidates that we may identify and successfully complete any clinical studies, including the manufacture of any such product candidates;

•our ability to pursue our areas of focus and any other additional programs we may advance;

•our ability to quickly leverage programs within our initial target indications and to progress additional programs to further develop our pipeline;

•the timing of our investigational new drug application (“IND”) submissions;

•the ability of our Prime Editing technology to address unmet medical needs in patients;

•the implementation of our strategic plans for our business, programs and technology;

•the scope and duration of protection we are able to establish and maintain for intellectual property rights covering our Prime Editing technology;

•developments related to our competitors and our industry;

•our ability to leverage the clinical, regulatory, and manufacturing advancements made by gene therapy and gene editing programs to accelerate our clinical trials and approval of product candidates;

•our ability to maintain collaborations or strategic relationships and the ability to identify and enter into future license agreements and collaborations;

•developments related to our Prime Editing technology;

•regulatory developments in the United States and foreign countries;

•our ability to attract and retain key scientific and management personnel;

•our estimates of our expenses, capital requirements, needs for additional financing;

•our expectations regarding the anticipated benefits of, and estimates related to, our strategic restructuring and reduction in force and our ability to implement and achieve the expected cost savings in connection therewith;

•our evaluation of strategic alternatives and potential partnership opportunities for PM359, including our ability to execute and realize the anticipated benefits of any strategic alternatives we may pursue;

•the effect of unfavorable macroeconomic conditions or market volatility resulting from national or global economic conditions or geopolitical developments, including rising inflation and capital market disruptions, changes in U.S. governmental agencies, new or increased international tariffs and retaliatory tariffs, trade protection measures, economic sanctions and economic slowdowns or recessions;

•our expectations regarding the anticipated timeline of our cash runway, and future financial performance ~~and our ability to continue as a going concern~~; and

•other risks and uncertainties, including those listed under the caption “Risk Factors.”

We may not actually achieve the plans, intentions or expectations disclosed in our forward-looking statements, and these statements may be affected by inaccurate assumptions or by known or unknown risks and uncertainties. You should not place undue reliance on our forward-looking statements. Actual results or events could differ materially from the plans, intentions and expectations disclosed in the forward-looking statements we make. We have included important factors in the cautionary statements included in this Quarterly Report on Form 10-Q and in subsequent SEC filings, particularly in the “Risk Factors” section, that we believe could cause actual results or events to differ materially from the forward-looking statements that we make. Unless otherwise disclosed, our forward-looking statements do not reflect the potential impact of any future acquisitions, mergers, dispositions, collaborations, joint ventures or investments we may make or enter into.

You should read this Quarterly Report on Form 10-Q and the documents that we reference herein and have filed as exhibits to our other filings with the Securities and Exchange Commission (the “SEC”) completely and with the understanding that our actual future results may be materially different from what we expect. The forward-looking statements contained in this Quarterly Report on Form 10-Q are made as of the date hereof, and we do not assume any obligation to update any forward-looking statements, whether as a result of new information, future events or otherwise after the date of such statements, except as required by applicable law.

This Quarterly Report on Form 10-Q also contains estimates, projections and other information concerning our industry, our business and the markets for our product candidates. Such information is inherently subject to uncertainties and actual events or circumstances may differ materially from events and circumstances that are assumed in this information. Unless otherwise expressly stated, we obtained statistical and other industry and market data from our own internal estimates and research, as well as from reports, industry publications and research, surveys, studies and similar data prepared by market research firms and other third parties, industry, medical and general publications, government data and similar sources. While we are not aware of any misstatements regarding any third-party information presented in this Quarterly Report on Form 10-Q, their estimates, in particular as they relate to projections, involve numerous assumptions, are subject to risks and uncertainties and are subject to change based on various factors, including those discussed under the section titled “Risk Factors” set forth in Part II, Item 1A of this Quarterly Report on Form 10-Q, if any, our Annual Report on Form 10-K that was filed with the SEC on February 28, 2025, and in other SEC filings.

PRIME MEDICINE, INC.

FORM 10-Q

FOR THE THREE AND SIX MONTHS ENDED ~~MARCH~~JUNE 3130, 2025

TABLE OF CONTENTS

		Page
PART I - FINANCIAL INFORMATION		5
Item 1.	Financial Statements (unaudited)	5
	Condensed Consolidated Balance Sheets as of March 31, 2025 and December 31, 2024	5
	Condensed Consolidated Balance Sheets as of June 30, 2025 and December 31, 2024	5
	Condensed Consolidated Statements of Operations and Comprehensive Loss for the Three Months Ended March 31, 2025 and, 2024	6
	Condensed Consolidated Statements of Operations and Comprehensive Loss for the Three and Six Months Ended June 30, 2025 and, 2024	6
	Condensed Consolidated Statements of Stockholders’ Equity for the Three Months Ended March 31, 2025 and, 2024	7
	Condensed Consolidated Statements of Stockholders’ Equity for the Three and Six Months Ended June 30, 2025 and, 2024	7
	Condensed Consolidated Statements of Cash Flows for the Three Months Ended March 31, 2025 and, 2024	9
	Condensed Consolidated Statements of Cash Flows for the Six Months Ended June 30, 2025 and, 2024	9
	Notes to Condensed Consolidated Financial Statements	11
Item 2.	Management’s Discussion and Analysis of Financial Condition and Results of Operations	24
Item 3.	Quantitative and Qualitative Disclosures About Market Risk	3132
Item 4.	Controls and Procedures	3133
PART II - OTHER INFORMATION		3334
Item 1.	Legal Proceedings	3334
Item 1A.	Risk Factors	3334
Item 2.	Unregistered Sales of Equity Securities and Use of Proceeds	3539
Item 3.	Defaults Upon Senior Securities	3539
Item 4.	Mine Safety Disclosure	3539
Item 5.	Other Information	3539
Item 6.	Exhibits	3640
	Signatures	3741

From time to time we may use our website, our X (formerly known as Twitter) account (@PrimeMedicine) or our LinkedIn profile at https://www.linkedin.com/company/prime-medicine to distribute material information. Our financial and other material information is routinely posted to and accessible on the Investors section of our website, available at www.primemedicine.com. Investors are encouraged to review the Investors section of our website because we may post material information on that site that is not otherwise disseminated by us. Information that is contained in and can be accessed through our website or our social media is not incorporated into, and does not form a part of, this Quarterly Report on Form 10-Q.

PART I - FINANCIAL INFORMATION

Item 1. Financial Statements (unaudited)

PRIME MEDICINE, INC.

CONDENSED CONSOLIDATED BALANCE SHEETS

(Unaudited)

(in thousands, except share and per share amounts)	~~March~~June 3130,2025		December 31,2024
Assets
Current assets:
Cash and cash equivalents	$	9153,~~877~~804	$	182,476
Short-term investments	4844,~~467~~539		2,998
Short-term investment — related party	3,~~912~~407		4,968
Collaboration receivable — related party	17120		—
Prepaid expenses	23,~~948~~032		6,777
Other current assets	16,~~544~~385		14,667
Total current assets	~~163~~121,~~765~~287		211,886
Property and equipment, net	2423,~~956~~828		24,404
Operating lease right-of-use assets	~~125~~120,~~380~~203		47,156
Restricted cash	1413,~~062~~691		14,062
Total assets	$	~~328~~279,~~163~~009	$	297,508
Liabilities and Stockholders’ Equity
Current liabilities:
Accounts payable	$	106,~~025~~378	$	11,351
Accrued expenses and other current liabilities	912,~~191~~158		15,904
Deferred revenue — related party	8,~~214~~315		7,092
Operating lease liability	67,~~858~~258		3,614
Total current liabilities	34,~~288~~109		37,961
Deferred revenue, net of current — related party	6059,~~658~~562		63,218
Operating lease liability, net of current	~~114~~112,~~293~~478		37,180
Research and development funding liability	12,000		6,000
Total liabilities	~~221~~218,~~239~~149		144,359
Commitments and contingencies
Stockholders’ equity
Common stock, par value of $0.00001 per share; 775,000,000 shares authorized; 131,160,842 and 131,160,842 shares issued and outstanding as of March 31, 2025 and December 31, 2024, respectively	2		2
Common stock, par value of $0.00001 per share; 775,000,000 shares authorized; 134,494,438 and 131,160,842 shares issued and outstanding as of June 30, 2025 and December 31, 2024, respectively	2		2
Additional paid-in capital	~~846~~852,~~039~~561		840,358
Accumulated other comprehensive loss	(1510)		1
Accumulated deficit	(~~739~~791,~~102~~693)		(687,212)
Total stockholders’ equity	~~106~~60,~~924~~860		153,149
Total liabilities and stockholders’ equity	$	~~328~~279,~~163~~009	$	297,508

The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

PRIME MEDICINE, INC.

CONDENSED CONSOLIDATED STATEMENTS OF OPERATIONS AND COMPREHENSIVE LOSS

(Unaudited)

			Three Months EndedMarch 31,
	Three Months EndedJune 30,				Six Months EndedJune 30,
(in thousands, except share and per share amounts)	2025		2024		2025		2024
Revenue:
Collaboration revenue — related party	$	1,115	$	—	$	12,~~454~~569	$	—
Collaboration revenue	—		—		—		591
Total revenue	1,115		—		12,~~454~~569		591
Operating expenses:
Research and development	41,375		43,071		4081,~~562~~937		3780,~~774~~845
General and administrative	13,117		12,601		1326,~~284~~401		1123,~~158~~759
Total operating expenses	54,492		55,672		53108,~~846~~338		48104,~~932~~604
Loss from operations	(53,377)		(55,672)		(52105,~~392~~769)		(48104,~~341~~013)
Other income:
Interest income	743		611		1,~~182~~925		~~676~~1,287
Accretion (amortization) of investments	530		1,486		~~339~~869		~~830~~2,316
Change in fair value of short-term investment — related party	(505)		(1,925)		(1,~~056~~561)		~~1,166~~(759)
Other income, net	18		39		3755		4281
Total other income, net	786		211		~~502~~1,288		2,~~714~~925
Net loss before income taxes	(52,591)		(55,461)		(51104,~~890~~481)		(45101,~~627~~088)
Provision for income taxes	—		134		—		~~(134)~~—
Net loss attributable to common stockholders	$	(52,591)	$	(55,327)	$	(51104,~~890~~481)	$	(45101,~~761~~088)
Net loss per share attributable to common stockholders, basic and diluted	$	(0.41)	$	(0.46)	$	(0.4081)	$	(0.4490)
Weighted-average common shares outstanding, basic and diluted	129,185,918		119,188,866		~~130~~128,~~884~~439,~~490~~349		~~104~~111,~~466~~827,~~178~~522
Comprehensive loss:
Net loss	$	(52,591)	$	(55,327)	$	(51104,~~890~~481)	$	(45101,~~761~~088)
Change in unrealized loss on investments, net of tax	5		24		(1611)		(8056)
Comprehensive loss	$	(52,586)	$	(55,303)	$	(51104,~~906~~492)	$	(45101,~~841~~144)

The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

PRIME MEDICINE, INC.

CONDENSED CONSOLIDATED STATEMENTS OF STOCKHOLDERS’ EQUITY

(Unaudited)

	Common Stock			AdditionalPaid-inCapital		Accumulated OtherComprehensiveLosses		AccumulatedDeficit		TotalStockholders’Equity
(in thousands, except share amounts)	Shares	Amount
Balance as of December 31, 2024	131,160,842	$	2	$	840,358	$	1	$	(687,212)	$	153,149
Stock-based compensation expense	—	—		5,681		—		—		5,681
Change in unrealized loss on investments, net of tax	—	—		—		(16)		—		(16)
Net loss	—	—		—		—		(51,890)		(51,890)
Balance as of March 31, 2025	131,160,842	$	2	$	846,039	$	(15)	$	(739,102)	$	106,924
Exercise of pre-funded warrants	3,199,984	—		—		—		—		—
Issuance of common stock under the employee stock purchase plan	133,612	—		197		—		—		197
Stock-based compensation expense	—	—		6,325		—		—		6,325
Change in unrealized loss on investments, net of tax	—	—		—		5		—		5
Net loss	—	—		—		—		(52,591)		(52,591)
Balance as of June 30, 2025	134,494,438	$	2	$	852,561	$	(10)	$	(791,693)	$	60,860

The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

PRIME MEDICINE, INC.

CONDENSED CONSOLIDATED STATEMENTS OF STOCKHOLDERS’ EQUITY

(Unaudited)

	Common Stock	AdditionalPaid-inCapital		Accumulated OtherComprehensiveLosses		AccumulatedDeficit			TotalStockholders’Equity
(in thousands, except share amounts)			Shares					Amount
Balance as of December 31, 2023		97,377,121		$	2	$	624,414		$	(15)	$	(491,330)	$	133,071
Issuance of common stock from public offering, net of issuance costs of $8.9 million		22,560,001		—		132,055			—		—		132,055
Issuance of pre-funded warrants, net of issuance costs of $1.2 million		—		—		18,800			—		—		18,800
Issuances of common stock under the employee stock purchase plan		74,488		—		436			—		—		436
Issuance of common stock under the employee stock purchase plan		74,488		—		436			—		—		436
Issuance of common stock upon exercise of stock options		9,664		—		36			—		—		36
Stock-based compensation expense		—		—		5,209			—		—		5,209
Change in unrealized loss on investments, net of tax		—		—		—			(80)		—		(80)
Net loss		—		—		—			—		(45,761)		(45,761)
Balance as of March 31, 2024		120,021,274		2		780,950			(95)		(537,091)		243,766
Issuance of common stock upon exercise of stock options		9,539		—		35			—		—		35
Stock-based compensation expense		—		—		8,091			—		—		8,091
Change in unrealized loss on investments, net of tax		—		—		—			24		—		24
Net loss		—		—		—			—		(55,327)		(55,327)
Balance as of June 30, 2024		120,030,813		$	2	$	789,076		$	(71)	$	(592,418)	$	196,589

The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

PRIME MEDICINE, INC.

CONDENSED CONSOLIDATED STATEMENTS OF CASH FLOWS

(Unaudited)

Three Months EndedMarch 31,

Six Months EndedJune 30,

(in thousands)

2025

2024

Cash flows used in operating activities:

Net loss

(51104,~~890~~481)

(45101,~~761~~088)

Adjustments to reconcile net loss to net cash used in operating activities

Stock-based compensation expense

512,~~681~~006

513,~~209~~300

Non cash lease expense

24,~~413~~761

37,~~348~~917

Depreciation expense

13,~~700~~564

12,~~315~~853

Change in fair value of short-term investment — related party

1,~~056~~561

~~(1,166)~~759

Amortization of premiums and discount on short-term investments

(~~164~~575)

(~~675~~2,079)

Deferred income taxes

—

~~134~~—

Changes in operating assets and liabilities:

Collaboration receivable — related party

(17120)

—

Prepaid expenses and other current assets

~~(789)~~652

(2,~~794~~519)

Accounts payable

(1,~~884~~461)

(59,~~880~~230)

Accrued expenses and other current liabilities

(61,~~655~~690)

(43,~~475~~939)

Accrued settlement payment — related party

—

(13,500)

Deferred revenue — related party

(12,~~438~~433)

—

Lease liabilities

(~~638~~2,053)

(35,~~462~~672)

Net cash used in operating activities

(4890,~~857~~269)

(67113,~~707~~198)

Cash flows used in investing activities:

Maturities of investments

314,~~000~~250

72110,~~500~~000

Purchases of investments

(4855,~~320~~227)

(~~107~~135,~~208~~912)

Purchases of property and equipment

(23,~~422~~994)

(24,~~324~~226)

Net cash used in investing activities

(4744,~~742~~971)

(3730,~~032~~138)

Cash flows provided by financing activities:

Proceeds from research and development funding liability

6,000

Proceeds from follow-on offering, net of issuance costs

—

132,055

Proceeds from issuance of pre-funded warrants, net of issuance costs

—

18,800

Proceeds from ESPP offerings

—197

436

Net proceeds from stock option exercises

—

3671

Net cash provided by financing activities

6,~~000~~197

157,~~327~~362

Net change in cash, cash equivalents, and restricted cash

(90129,~~599~~043)

5214,~~588~~026

Cash, cash equivalents, and restricted cash at beginning of period

196,538

55,070

Cash, cash equivalents, and restricted cash at end of period

~~105~~67,~~939~~495

~~107~~69,~~658~~096

The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

PRIME MEDICINE, INC.

CONDENSED CONSOLIDATED STATEMENTS OF CASH FLOWS

(Unaudited)

	Three Months EndedMarch 31,
	Six Months EndedJune 30,
(in thousands)	2025		2024
Reconciliation of cash, cash equivalents and restricted cash:
Cash, cash equivalents, and restricted cash at end of period	$	~~105~~67,~~939~~495	$	~~107~~69,~~658~~096
Less: restricted cash	1413,~~062~~691		13,496
Total cash, and cash equivalents	$	9153,~~877~~804	$	9455,~~162~~600
Supplemental cash flow information:
Right-of-use assets obtained in exchange for new operating lease liabilities	$	83,757	$	44,935
Decrease in right-of-use assets due to lease termination	$	3,120	$	—
Supplemental disclosure of non-cash investing and financing activities:
Purchases of property and equipment included in accounts payable and accrued expenses	$	~~985~~148	$	~~833~~355

The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

PRIME MEDICINE, INC.

NOTES TO CONDENSED CONSOLIDATED FINANCIAL STATEMENTS

(Unaudited)

1.Nature of the Business and Basis of Presentation

Prime Medicine, Inc., together with its consolidated subsidiary (the “Company”) is a biotechnology company committed to delivering a new class of differentiated one-time curative genetic therapies. The Company is deploying Prime Editing technology, which it believes is a versatile, precise, and efficient gene editing technology. The Company was incorporated in the State of Delaware in September 2019. Its principal offices are in Cambridge, Massachusetts.

Liquidity and Capital Resources

Since its inception, the Company has devoted substantially all of its resources to building its Prime editing platform and advancing development of its portfolio of programs, establishing and protecting its intellectual property, conducting research and development activities, organizing and staffing the company, business planning, raising capital and providing general and administrative support for these operations. To date, the Company has funded its operations primarily with proceeds from sales of preferred stock, ~~and~~ from public offerings, and private placement of its common stock, and through payments from ~~our~~its collaboration partners.

~~Since~~In ~~its~~August ~~inception~~2025, the Company ~~has~~issued ~~incurred~~and ~~substantial~~sold ~~losses and~~43,700,000 asshares of ~~March 31,~~its ~~2025~~common stock, ~~the~~including ~~Company~~5,700,000 ~~had~~shares anpursuant ~~accumulated~~to ~~deficit~~the exercise of ~~$739.1~~the ~~million.~~underwriters’ ~~The~~option ~~Company expects~~ to ~~generate~~purchase ~~operating~~additional ~~losses~~shares, ~~and~~at ~~negative~~a ~~operating~~price ~~cash~~to ~~flows~~the ~~for~~public ~~the~~of ~~foreseeable~~$3.30 ~~future~~per share. As a result of ~~March 31,~~the ~~2025~~offering, the Company ~~maintains~~received ~~cash~~approximately $138.2 million in net proceeds, ~~cash~~after ~~equivalents~~deducting underwriting discounts, commissions and ~~investments~~estimated offering costs of $~~144~~6.30 million.

~~Going~~Since ~~Concern~~its inception, the Company has incurred substantial losses and, as of June 30, 2025, the Company had an accumulated deficit of $791.7 million. The Company expects to generate operating losses and negative operating cash flows for the foreseeable future. The Company further expects that its cash, cash equivalents, and investments as of June 30, 2025 of $101.8 million, along with the $138.2 million in net proceeds received from the sale of common stock in August 2025 discussed above, will be sufficient to fund its operations for at least the next twelve months from the date of issuance of these financial statements.

In accordance with Accounting Standards Codification (“ASC”) 205-40, Going Concern, (“ASC 205-40”) the Company evaluated whether there are conditions and events, considered in the aggregate, that raise substantial doubt about the Company’s ability to continue as a going concern within one year after the date on which this Quarterly Report on Form 10-Q is filed. Based on the Company's cash, cash equivalents, short-term investments, and related party short-term investments as of March 31, 2025, the Company's current and forecasted level of operations, and its forecasted cash flows, management has determined that substantial doubt exists about the Company’s ability to continue as a going concern for 12 months from the date these condensed consolidated financial statements are issued. The Company’s ability to continue as a going concern is dependent upon its ability to obtain the necessary financing to meet its obligations and repay its liabilities arising from normal business operations when they come due, and to generate profitable operations in the future. Management plans to provide for the Company’s capital requirements through financing or other transactions, and selling shares under the Company's “at the market offering” program. There can be no assurance that the Company will be able to raise additional capital to fund operations with terms acceptable to the Company, or at all. Because certain elements of management’s plans to mitigate the conditions that raised substantial doubt about the Company’s ability to continue as a going concern are outside of the Company’s control, including the ability to raise capital through an equity or other financing, those elements cannot be considered probable according to ASC 205-40, and therefore cannot be considered in the evaluation of mitigating factors.

The condensed consolidated financial statements as of March 31, 2025 have been prepared under the assumption that the Company will continue as a going concern for the next 12 months and that contemplates the realization of assets and satisfaction of liabilities and commitments in the normal course of business. The Company’s ability to continue as a going concern is dependent upon its uncertain ability to obtain additional capital, reduce expenditures and/or execute on its business plan. These condensed consolidated financial statements do not include any adjustments that might result from the outcome of this uncertainty.

Risks and Uncertainties

The Company is subject to risks and uncertainties common to early stage companies in the biotechnology industry, including, but not limited to, completing preclinical studies and clinical trials, obtaining regulatory approval for product candidates, market acceptance of products, development by competitors of new technological innovations, dependence on key personnel, the ability to attract and retain qualified employees, reliance on third-party organizations, protection of proprietary technology, compliance with government regulations, and the ability to raise additional capital to fund operations. The Company’s product candidates currently under development will require significant additional research and development efforts, including extensive preclinical and clinical testing and regulatory approval prior to commercialization. These efforts require significant amounts of additional capital, adequate personnel and infrastructure, and extensive compliance-reporting capabilities. Even if the Company’s development efforts are successful, it is uncertain when, if ever, the Company will realize significant revenue from product sales.

The Company will need to raise additional capital to support its continuing operations and to pursue its growth strategy. Until such time as the Company can generate significant revenue from product sales, if ever, it expects to finance its operations through a combination of private and public equity offerings, debt financings, collaborations, strategic alliances, ~~and~~ licensing, or other arrangements with third parties, or other similar transactions. The Company may be unable to raise additional capital or enter into such other agreements when needed on favorable terms or at all. The inability to raise capital as and when needed would have a negative impact on the Company’s financial ~~condition and its ability to pursue its business strategy. The Company will need to generate significant revenue to achieve profitability, and it may never do so.~~

condition and its ability to pursue its business strategy. The Company will need to generate significant revenue to achieve profitability, and it may never do so.

Basis of Presentation

The accompanying condensed consolidated financial statements reflect the operations of the Company and its wholly-owned subsidiary. Intercompany balances and transactions have been eliminated in consolidation. The accompanying condensed consolidated financial statements have been prepared in conformity with generally accepted accounting principles in the United States of America (“U.S. GAAP”). Any reference in these notes to applicable guidance is meant to refer to the authoritative GAAP as found in the ASC and Accounting Standards Updates (“ASU”) of the Financial Accounting Standards Board (“FASB”).

The accompanying condensed consolidated financial statements of Prime Medicine, Inc. are unaudited. The unaudited interim condensed consolidated financial statements have been prepared on the same basis as the audited annual consolidated financial statements and, in the opinion of management, reflect all adjustments, which include only normal recurring adjustments, necessary for the fair statement of the Company’s financial position as of ~~March~~June 3130, 2025, the results of its operations for the three and six months ended ~~March~~June 3130, 2025 and 2024, the condensed consolidated statements of stockholders’ equity for the three and six months ended ~~March~~June 3130, 2025 and 2024, and its cash flows for the ~~three~~six months ended ~~March~~June 3130, 2025 and 2024. The financial data and other information disclosed in these notes related to the three and six months ended ~~March~~June 3130, 2025 and 2024 are also unaudited. The results for the three and six months ended ~~March~~June 3130, 2025 are not necessarily indicative of results to be expected for the year ending December 31, 2025, any other interim periods, or any future year or period. The condensed consolidated balance sheet data as of December 31, 2024 was derived from our audited financial statements, but does not include all disclosures required by U.S. GAAP. These interim financial statements should be read in conjunction with the audited financial statements as of and for the year ended December 31, 2024, and notes thereto, which are included in the Company’s Annual Report on Form 10-K that was filed with the Securities and Exchange Commission (the “SEC”) on February 28, 2025.

Change in Presentation

For the three months ended March 31, 2025, “Other income, net” as presented on the condensed consolidated statements of operations and comprehensive loss was disaggregated into “Accretion (amortization) of investments,” “Interest income,” and “Other income, net.” This presentation has been conformed for all previous periods presented and had no impact on previously reported results.

2.Summary of Significant Accounting Policies

The Company’s significant accounting policies are disclosed in Note 2, Summary of significant accounting policies, in the audited consolidated financial statements for the year ended December 31, 2024, and notes thereto, included in the Company’s Annual Report on Form 10-K that was filed with the SEC on February 28, 2025. Since the date of those financial statements, there have been no material changes to its significant accounting policies, except as noted below.

Use of Estimates

The preparation of the Company’s condensed consolidated financial statements in conformity with U.S. GAAP requires management to make estimates and assumptions that affect the reported amounts of assets and liabilities and the disclosure of contingent assets and liabilities at the date of the condensed consolidated financial statements and the reported amounts of revenues and expenses during the reporting periods. Significant estimates and assumptions reflected within these condensed consolidated financial statements include, but are not limited to, the valuation of the Company’s common stock and stock-based awards. The Company bases its estimates on historical experience, known trends and other market-specific or other relevant factors that it believes to be reasonable under the circumstances. On an ongoing basis, management evaluates its estimates, as there are changes in circumstances, facts and experience. Actual results may differ materially from those estimates or assumptions.

Segment Information

Under ASC 280, Segment Reporting, operating segment is a component of a public entity that 1) engages in business activities from which it may recognize revenues and incur expenses, 2) its operating results are regularly reviewed by the entity’s chief operating decision maker (“CODM”) to make decisions about resource allocation or performance assessment, and 3) its discrete financial information is available. The Company operates and manages its business as a single segment for the purposes of assessing performance and making operating decisions.

Our chief executive officer, who is the CODM, manages and allocates resources to the operations of our company on a total company basis by assessing the overall level of resources available and how to best deploy these resources across functions and research and development projects that are in line with our long-term company-wide strategic goals. In making these decisions, the CODM uses consolidated financial information for purposes of evaluating performance, forecasting future period financial results, allocating resources and setting incentive targets. The CODM performs this assessment based on the Company’s consolidated net loss. Through this analysis, the CODM assesses performance by comparing actual net loss verses the budget, and then decides how to allocate resources to invest in the Company’s research and development programs. The measure of segment assets is reported on the consolidated balance sheets as total assets.

The following table contains additional information on our consolidated net loss, including significant segment expenses:

			Three Months Ended March 31,
	Three Months Ended June 30,				Six Months Ended June 30,
(in thousands)	2025		2024		2025		2024
Total revenue	$	1,115	$	—	$	12,~~454~~569	$	591
Operating expenses:
Research and development expenses
Personnel expenses	14,448		16,198		1429,~~800~~248		1531,~~007~~205
Facility related	13,765		11,302		1024,~~969~~734		718,~~334~~636
Research costs	8,116		11,390		1119,~~036~~152		1123,~~619~~009
General and administrative expenses:
Personnel expenses	7,082		6,956		714,~~156~~238		512,~~884~~840
Other segment items (1)	11,081		9,826		920,~~885~~966		918,~~088~~914
Total operating expenses	54,492		55,672		53108,~~846~~338		48104,~~932~~604
Total other income, net	786		211		~~502~~1,288		2,~~714~~925
Provision for income taxes	—		134		—		~~(134)~~—
Net loss	$	(52,591)	$	(55,327)	$	(51104,~~890~~481)	$	(45101,~~761~~088)

(1) Other segment items consist of professional and consultant fees, license and intellectual property fees, general and administrative facility costs, and settlement costs.

Recently Issued Accounting Pronouncements Not Yet Adopted

In December 2023, the FASB issued ASU 2023-09, Income Taxes (Topic 740): Improvements to Income Tax Disclosures, which requires enhanced income tax disclosures, including specific categories and disaggregation of information in the effective tax rate reconciliation, disaggregated information related to income taxes paid, income or loss from continuing operations before income tax expense or benefit, and income tax expense or benefit from continuing operations. This guidance is effective for annual periods beginning after December 15, 2024, with early adoption permitted. The Company is currently in the process of evaluating the impact of this pronouncement on its related disclosures.

In November 2024, the FASB issued ASU 2024-03, Disaggregation of Income Statement Expenses, which requires more detailed information about specified categories of expenses included in certain expense captions presented on the face of the income statement. This ASU is effective for fiscal years beginning after December 15, 2026, and for interim periods within fiscal years beginning after December 15, 2027. Early adoption is permitted. The amendments may be applied either (1) prospectively to financial statements issued for reporting periods after the effective date of this ASU or (2) retrospectively to all prior periods presented in the financial statements. The Company is currently evaluating the impact this ASU will have on its disclosures.

Other accounting standards that have been issued by the FASB or other standards-setting bodies that do not require adoption until a future date are not expected to have a material impact on the Company’s financial statements upon adoption.

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3.Fair Value Measurements and Investments

The following tables present the Company’s fair value hierarchy for its assets that are measured at fair value on a recurring basis and indicate the level of the fair value hierarchy utilized to determine such fair value:

	As of March 31, 2025:
	As of June 30, 2025:
(in thousands)	Level 1		Level 2		Level 3		Total
Cash equivalents:
Money market funds	$	—	$	6240,~~610~~748	$	—	$	6240,~~610~~748
U.S. Treasury and government securities	—		20,797		—		20,797
Corporate debt securities	—		6,776		—		6,776
Corporate debt securities	—		7,783		—		7,783
U.S. Treasury and government securities	—		5,216		—		5,216
Short-term investments:
U.S. Treasury and government securities	—		3836,~~583~~347		—		3836,~~583~~347
Corporate debt securities	—		98,~~884~~192		—		98,~~884~~192
Related party short-term investment:
Beam equity securities	3,~~912~~407		—		—		3,~~912~~407
Total cash equivalents and investments	$	3,~~912~~407	$	~~139~~97,~~657~~279	$	—	$	~~143~~100,~~569~~686


	As of December 31, 2024:
(in thousands)	Level 1		Level 2		Level 3		Total
Cash equivalents:
Money market funds	$	—	$	178,212	$	—	$	178,212
Corporate debt securities	—		3,793		—		3,793
Short-term investment:
U.S. Treasury and government securities	—		2,998		—		2,998
Related party short-term investment:
Beam equity securities	4,968		—		—		4,968
Total cash equivalents and investments	$	4,968	$	185,003	$	—	$	189,971

The Company classifies its investments as short-term based on each instrument’s underlying contractual maturity date. The fair value of investments classified as Level 2 are valued using observable inputs to quoted market prices, benchmark yields, reported trades, broker/dealer quotes or alternative pricing sources with reasonable levels of price transparency.

Investments in Debt Securities

Unrealized gains and losses of investments in debt securities consisted of the following:

	As of March 31, 2025:
	As of June 30, 2025:
(in thousands)	Amortized Cost		Unrealized Gains		Unrealized Losses		Fair Value
Short-term investments in debt securities:
U.S. Treasury and government securities	$	3836,~~595~~355	$	—	$	(128)	$	3836,~~583~~347
Corporate debt securities	98,~~887~~194		—		(32)		98,~~884~~192
Total short-term investments in debt securities	$	4844,~~482~~549	$	—	$	(1510)	$	4844,~~467~~539

1514


	As of December 31, 2024:
(in thousands)	Amortized Cost		Unrealized Gains		Unrealized Losses		Fair Value
Short-term investments:
U.S. Treasury and government securities	$	2,997	$	1	$	—	$	2,998
Total short-term investments in debt securities	$	2,997	$	1	$	—	$	2,998

The contractual maturities of the Company’s investments in debt securities ~~held~~ were as follows:

(in thousands)

~~March~~June 3130,2025

December 31,2024

Due within one year

4844,~~467~~539

2,998

Marketable securities in unrealized loss positions consisted of the following:

	As of March 31, 2025:
	As of June 30, 2025:
(in thousands, except number of securities)	Number of Securities	Fair Value		Gross Unrealized Losses
Investments in continuous loss position for less than 12 months:
U.S. Treasury and government securities	1112	$	3836,~~583~~347	$	(128)
Corporate debt securities	109	$	98,~~884~~192	$	(32)

Based on factors such as historical experience, market data, issuer-specific factors, and current economic conditions, the Company did not record an allowance for credit losses as of ~~March~~June 3130, 2025 related to these investments. Further, given the lack of significant change in the credit risk, the Company does not consider these investments to be impaired.

4.Property and Equipment, Net

Property and equipment, net consisted of the following:

(in thousands)

~~March~~June 3130,2025

December 31,2024

Property and equipment:

Laboratory equipment

2829,~~093~~377

27,343

Leasehold improvement

87,~~269~~911

5,136

Furniture and Fixture

1,~~800~~864

1,075

Computer hardware and software

1,~~010~~122

869

Construction in progress

~~1,081~~230

3,578

Total property and equipment

40,~~253~~504

38,001

Less: Accumulated depreciation

(1516,~~297~~676)

(13,597)

Total property and equipment, net

2423,~~956~~828

24,404

Depreciation expense related to property and equipment is as follows:

			Three Months Ended March 31,
	Three Months Ended June 30,				Six Months Ended June 30,
(in thousands)	2025		2024		2025		2024
Depreciation Expense	$	1,864	$	1,538	$	13,~~700~~564	$	12,~~315~~853

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5.Accrued Expenses and Other Current Liabilities

Accrued expenses and other current liabilities consisted of the following:

(in thousands)	~~March~~June 3130,2025		December 31,2024
Accrued expenses and other current liabilities
Employee compensation and benefits	$	45,~~179~~818	$	8,976
Research and clinical costs	13,~~977~~660		907
Facility related	317		2,811
License fee	1,562		1,938
License fee	—		1,938
Facility related	1		2,811
Other	21,~~718~~117		1,272
Total accrued expenses and other current liabilities	$	912,~~191~~158	$	15,904

6.Leases

The Company leases office and laboratory space under various non-cancelable operating leases. The Company’s significant lease agreements are disclosed in Note 9, Leases, in the audited consolidated financial statements for the year ended December 31, 2024, and notes thereto, included in the Company’s Annual Report on Form 10-K that was filed with the SEC on February 28, 2025. Since the date of those financial statements, there have been no changes to the Company’s significant agreements except as described below.

60 First Street, Cambridge, Massachusetts Lease

In November 2021, the Company entered into a lease for three floors of office and laboratory space in Cambridge, Massachusetts, with rent commencing in March 2024, subject to any credits pursuant to the terms of the lease (the “60 First Street Lease”). Subsequent to the initial non-cancelable term of the lease of ten years, the Company has an option to extend the lease for an additional period of ten years with the rent during the extension term being the then fair market rent. The Company secured the lease with a $13.1 million security deposit, which was recorded as restricted cash on the condensed consolidated balance sheets as of ~~March~~June 3130, 2025 and December 31, 2024.

Accounting Considerations

The Company determined that the lease contained three separate lease components, each of which represents a right of use that the Company can benefit from on its own and neither of which are highly dependent nor highly related to each other. The Company allocated the consideration among the three lease components based on their relative fair market values.

In accordance with ASC 842, Leases, the lease commenced for one of the lease components in March 2024 and the Company recorded a right-of-use asset of $44.9 million, and a corresponding lease liability of $33.6 million on the lease commencement date; this included a reclass of $11.3 million from prepaid expenses to right-of-use asset related to build out costs which were determined to be owned by the lessor. In March 2025, the Company determined that the lease commenced on the remaining two lease components and recorded right-of-use assets of $76.8 million, and a corresponding lease liability of $78.6 million on the lease commencement date. As the exercise of the option to extend the lease term is not reasonably certain, the Company will recognize lease expense for all lease components through February 2034.

In June 2024, the Company filed a complaint in Massachusetts Superior Court against NW Cambridge Property Owner, LLC (“NWC”), the Company’s landlord at 60 First Street, alleging that NWC had breached the 60 First Street Lease by, among other reasons, refusing to provide the Company with certain rental credits for the late delivery of the leased space. While this lawsuit is pending, the Company ~~has~~ continued to make the disputed payments under the 60 First Street Lease under protest. InThe ~~March~~disputed ~~2025,~~payments ~~the~~are ~~Court~~recorded ~~granted~~within ~~NWC’s~~other ~~motion~~current ~~for~~assets ~~judgment~~ on the ~~pleadings, dismissing the~~ Company’s ~~claims~~condensed ~~against~~consolidated ~~NWC~~balance sheets. InTotal ~~April~~payments ~~2025,~~made ~~the~~under ~~Company~~dispute ~~filed~~was a$13.9 ~~notice~~million, ofand ~~appeal~~$10.8 ofmillion ~~the~~as ~~Court’s~~of ~~order~~June 30, ~~granting~~2025 ~~the motion for judgment on the pleadings~~ and ~~served a motion for leave to amend its complaint on NWC~~December 31, ~~which is currently pending. Pending a ruling on the amended complaint~~2024, ~~the~~ respectively.

1716

disputed payments are recorded within other current assets on the Company’s condensed consolidated balance sheets. Total payments made under dispute was $13.9 million, and $10.8 million as of March 31, 2025 and December 31, 2024, respectively.

Arsenal Street, Watertown, Massachusetts Leases

In August 2024, the Company entered into the third amendment to its existing lease for approximately 16,000 square feet of combined laboratory and office space at 480 Arsenal Street, Watertown, Massachusetts (the “480 Arsenal Amendment”). In September 2024, the Company entered into a new lease for approximately 48,500 square feet of combined laboratory and office space at 500 Arsenal Street, Watertown, Massachusetts (the “500 Arsenal Lease”). The landlords of the spaces at 480 Arsenal Street and 500 Arsenal Street are affiliates.

The 480 Arsenal Amendment provides the Company with an additional 9,400 square feet of combined laboratory and office space (the “Expansion Space”) at no additional cost and also provides an early termination date for the existing space and the Expansion Space as the later of: 1) the date occurring 150 days after the lease commencement of 500 Arsenal Lease, or 2) 30 days after substantial completion, as defined in the 500 Arsenal Lease, of tenant improvements at 500 Arsenal Street. IfThe ~~the 500 Arsenal Lease is terminated prior to its~~ lease ~~commencement such that the lease commencement never occurs, the Company will begin paying lease payments on the Expansion Space at the current rate~~ for ~~its existing~~ space at 480 Arsenal Street, ~~and~~including the ~~early~~Expansion ~~termination~~Space, ~~provisions~~terminated ofon ~~the~~April ~~480~~30, ~~Arsenal Amendment become null and void~~2025.

The 500 Arsenal Lease term commenced in December 2024 with a base term of 11 months. In December 2024, the Company began constructing improvements to the space, the construction for which was completed in 2025. Subsequent to the base term, the Company has an option to extend the lease through August 2028, which it exercised in February 2025. The Company secured the lease with a $0.6 million security deposit, which is recorded as restricted cash on the condensed consolidated balance sheets as of ~~March~~June 3130, 2025. The 500 Arsenal Lease also provides a tenant improvement allowance of $2.4 million and an additional tenant improvement allowance of $1.2 million, which the Company would be obligated to repay to the landlord. The Company did not use the additional tenant improvement allowance.

Accounting Considerations

As the 480 Arsenal Amendment and the 500 Arsenal Lease meet the criteria for combining contracts under ASC 842, the Company determined that both 480 Arsenal Amendment and the 500 Arsenal Lease are modifications to its existing lease at 480 Arsenal Street. Within the combined contract the Company identified two separate lease components, each of which represents a right of use that the Company can benefit from on its own and none which are neither highly dependent nor highly related to the other. The Company allocated the consideration under the combined contract among the two lease components based on their relative fair market value. In calculating the allocable consideration and the fair market value of each lease component, the Company determined it is probable that it will exercise the option to extend the lease term provided under the 500 Arsenal Lease.

For accounting purposes, as the construction of the lessor assets were completed, the Company determined that the 500 Arsenal Lease commenced in March 2025 and recorded right-of-use assets of $7.0 million, and a corresponding lease liability of $5.5 million on the lease commencement date. Concurrently, the Company also determined that the termination of its leased space at 480 Arsenal Street was reasonably certain, and the Company recorded a $3.1 million reduction to the Company’s operating lease liability and its operating lease right-of-use asset on the condensed consolidated balance sheet.

1817

The table below reconciles the undiscounted future annual lease payments to the total operating lease liabilities recorded in the condensed consolidated balance sheet as of ~~March~~June 3130, 2025:

(in thousands)	UndiscountedAmounts
Undiscounted lease payments:
Remaining in 2025	$	1510,~~856~~494
2026	21,703
2027	22,355
2028	22,294
2029	21,478
Thereafter	96,601
Total undiscounted lease payments	~~200~~194,~~287~~925
Less: imputed interest	(7975,~~136~~189)
Total operating lease liability	$	~~121~~119,~~151~~736

7.Stockholder’s Equity

Common Stock

Under the Company’s Third Amended and Restated of Certificate of Incorporation, as amended, the Company’s common stock had a par value of $0.~~0001~~00001 and each share of common stock entitles the holder to one vote on all matters submitted to the stockholders for a vote. The holders of common stock are entitled to receive dividends, if any, as declared by the Company’s Board of Directors (the “Board of Directors”).

Pre-funded Warrants

In February 2024, the Company sold pre-funded warrants to purchase 3,200,005 shares of common stock at a public offering price of $6.24999 per pre-funded warrant, which represents the per share public offering price of each share of common stock less the $0.00001 per share exercise price for each pre-funded warrant. Subject to certain requirements, the pre-funded warrants can be exercised by the holder at any time. ~~As of March 31, 2025, there have not been any exercises of the pre-funded warrants.~~

In May 2025, the holder exercised all 3,200,005 of its pre-funded warrants on a cashless basis and received 3,199,984 shares of the Company’s common stock. Following this exercise, the Company has no pre-funded warrants outstanding.

August 2025 Offering

In August 2025, the Company issued and sold 43,700,000 shares of its common stock, including 5,700,000 shares pursuant to the exercise of the underwriters’ option to purchase additional shares, at a price to the public of $3.30 per share. As a result of the offering, the Company received approximately $138.2 million in net proceeds, after deducting underwriting discounts, commissions and offering costs of approximately $6.0 million.

At-The-Market Equity Program

In November 2023, the Company entered into Open Market Sale AgreementSM (the “Sales Agreement”) with Jefferies LLC, acting as the Company’s agent and/or principal (the “Sales Agent”), with respect to an “at the market offering” program under which the Company may, from time to time, at its sole discretion, issue and sell shares of its common stock having an aggregate offering price of up to $300.0 million through the Sales Agent. ~~As of March 31, 2025, there have been no sales of common stock pursuant to the Sales Agreement.~~

Effective July 30 2025, the Company terminated the sales agreement prospectus (the “ATM Prospectus”) filed with the shelf registration statement on Form S-3 (File No. 333-275321) and related to the shares of the Company’s common stock issuable pursuant to the Sales Agreement. As a result, the Company will not make any sales of its common stock pursuant to the Sales Agreement, unless and until a new prospectus, prospectus supplement or a new

registration statement is filed. Other than the termination of the ATM Prospectus, the Sales Agreement remains in full force and effect.

As of June 30, 2025 and as of the date these condensed consolidated financial statements are issued, the Company has not sold any shares of common stock under the ATM Prospectus.

8.Stock-Based Compensation

2019 ~~Equity~~Stock ~~Incentive~~Option and Grant Plan

The Company’s 2019 Stock Option and Grant Plan (the “2019 Plan”) provides for the Company to grant incentive stock options, non-qualified stock options, unrestricted stock awards, restricted stock awards and other stock-based awards to the officers, employees, consultants and other key persons of the Company. The 2019 Plan was administered by the Board of Directors, or at the discretion of the Board of Directors, by a committee of the Board of Directors. The exercise prices, vesting and other restrictions are determined at the discretion of the Board of Directors, or its committee if so delegated.

In October 2022, in connection with the closing of the Company’s initial public offering (“IPO”), the Board of Directors determined that no further awards would be granted under the 2019 Plan.

2022 Stock Option and Incentive Plan

On February 9, 2022, the Board of Directors adopted, and on October 10, 2022, the Company’s stockholders approved, the 2022 Stock Option and Incentive Plan (the “2022 Plan”), which became effective on October 18, 2022. The 2022 Plan allows the Company to make equity-based and cash-based incentive awards to its officers, employees, directors, and consultants. The 2022 Plan provides for the grant of incentive stock options, non-qualified stock options, stock appreciation rights, restricted stock awards, restricted stock units and other stock-based awards.

The shares of common stock underlying any awards under the 2022 Plan and the 2019 Plan that are forfeited, cancelled, held back upon exercise or settlement of an award to satisfy the exercise price or tax withholding, reacquired by the Company prior to vesting, satisfied without the issuance of stock, expire, or are otherwise terminated (other than by exercise) will be added back to the shares of common stock available for issuance under the 2022 Plan. The number of shares reserved and available for issuance under the 2022 Plan increased on January 1, 2023 and will increase on each January 1 hereafter, by five percent of the outstanding number of shares of common stock on the immediately preceding December 31 or such lesser number of shares as determined by the Compensation Committee of the Board of Directors. On January 1, 2025, the annual increase resulted in an additional 6,558,042 shares authorized being added to the 2022 Plan. As of ~~March~~June 3130, 2025, the Company had 28,080,152 shares reserved under the 2022 Plan and the 2019 Plan, and 119,~~689~~478,~~462~~749 shares available for issuance under the 2022 Plan.

2022 Employee Stock Purchase Plan

On February 9, 2022, the Board of Directors adopted, and on October 10, 2022, the Company’s stockholders approved, the 2022 Employee Stock Purchase Plan (the “2022 ESPP”), which became effective on October 18, 2022.

The number of shares of common stock that may be issued under the 2022 ESPP cumulatively increased beginning on January 1, 2023 and shall increase on each January 1 hereafter through January 1, 2032, by the least of (i) 971,350 shares of common stock, (ii) one percent of the outstanding number of shares of common stock on the immediately preceding December 31, or (iii) such number of shares of common stock as determined by the administrator of the 2022 ESPP. There was no annual increase for the 2022 ESPP on January 1, 2025. As of ~~March~~June 3130, 2025, the Company had 1,~~753~~619,~~191~~579 shares available for issuance under the 2022 ESPP.

During the ~~three~~six months ended ~~March~~June 3130, 2025, the Company issued no133,612 shares of the Company’s common stock under the 2022 ESPP.

Stock Options

The following table summarizes the Company’s stock option activity for the ~~three~~six months ended ~~March~~June 3130, 2025:

	Number of Shares	Weighted-Average Exercise Price
Outstanding at December 31, 2024	11,410,691	$	9.08
Granted	47,~~307~~531,~~544~~444	21.4094
Exercised	—	—
Cancelled or forfeited	(~~389~~2,~~275~~102,462)	87.6604
Outstanding at March 31, 2025	15,328,960	$	7.21
Outstanding at June 30, 2025	16,839,673	$	6.14
Options vested and exercisable at March 31, 2025	6,026,956	$	8.66
Options vested and exercisable at June 30, 2025	7,106,428	$	8.45
Options vested and expected to vest at March 31, 2025	15,328,960	$	7.21
Options vested and expected to vest at June 30, 2025	16,839,673	$	6.14

As of ~~March~~June 3130, 2025, there was $4030.05 million of total unrecognized compensation cost related to time-based unvested stock options. The Company expects to recognize such amount over a remaining weighted-average period of 2.89 years.

Performance-Based Stock Options

The following table summarizes the Company’s performance-based stock option activity for the ~~three~~six months ended ~~March~~June 3130, 2025:

	Number of Shares	Weighted-AverageGrant Date Fair Value
Outstanding at December 31, 2024	1,061,730	$	7.31
Granted	—700,000	—1.34
Exercised	—	—
Cancelled or forfeited	—	—
Outstanding at March 31, 2025	1,061,730	$	7.31
Outstanding at June 30, 2025	1,761,730	$	4.94
Vested and exercisable at March 31, 2025	590,230	$	7.07
Vested and exercisable at June 30, 2025	845,646	$	6.87

As of ~~March~~June 3130, 2025, there was $32.01 million of total unrecognized compensation cost related to performance-based stock options.

Repricing of Certain Outstanding Stock Options

On June 22, 2025, the Board of Directors approved, and on August 1, 2025 (the “Repricing Date”), the Company’s stockholders approved, a one-time repricing (the “Option Repricing”) of certain outstanding stock options granted to, and held by, certain of the Company’s current employees, including its executive officers, and members of the Board of Directors through the Repricing Date (collectively, the “Eligible Optionholders”), under the 2019 Plan and 2022 Plan (collectively, the “Plans”). The Option Repricing impacted up to an aggregate of 8,285,387 shares of common stock (such options, the “Eligible Options”) that have exercise prices in excess of $4.04 (the “Repriced Exercise Price”), which was the closing trading price per share of the Company’s common stock on The Nasdaq Global Market on the Repricing Date.

Effective as of the Repricing Date, the per share exercise price of each Eligible Option held by an Eligible Optionholder on the Repricing Date was automatically reduced to the Repriced Exercise Price. Under the terms of the Option Repricing, a Repriced Option will revert to its original exercise price per share if:

i.exercised prior to the 18-month anniversary of the Repricing Date for Repriced Options held by members of the Board of Directors or executive officers of the Company, as applicable, or the one-year anniversary of the Repricing Date for Repriced Options held by all other employees of the Company (each, a “Retention Date”);

ii.an Eligible Optionholder’s Service Relationship (as defined in the Plans) is terminated by the Company for Cause (as defined in the applicable award agreement) prior to the applicable Retention Date; or

iii.an Eligible Optionholder resigns from the Company (including as a member of the Board of Directors) for any reason prior to the applicable Retention Date, other than described below.

Notwithstanding the foregoing, each Repriced Option shall retain the Repriced Exercise Price, to the extent it has not otherwise reverted to its original exercise price per share in accordance with the foregoing, and the ability to exercise such Repriced Option may be accelerated to earlier than the applicable Retention Date in the event of:

i.a Sale Event (as defined in the Plans) prior to the applicable Retention Date;

ii.the termination of the Eligible Optionholder’s Service Relationship by the Company without Cause or for “good reason,” or resignation by the Eligible Optionholder for “good reason,” to the extent provided for in the Eligible Optionholder’s employment agreement, offer letter or severance plan (as applicable); or

iii.the Eligible Optionholder’s death or disability (as determined in accordance with the Plans, as applicable)..

Except as modified by the Option Repricing, all other terms and conditions of the Repriced Options, including, without limitation, any provisions with respect to vesting and term of the Repriced Options, will remain in full force and effect. The Company is in the process of evaluating the accounting impact of the Option Repricing.

Performance-Based Restricted Common Stock Awards

The Company awarded restricted common stock to employees and non-employees under its 2019 Plan. The following table summarizes the Company’s performance-based restricted common stock activity for the ~~three~~six months ended ~~March~~June 3130, 2025:

	Number of Shares	Weighted-Average Grant-Date Fair Value
Outstanding at December 31, 2024	3,472,~~543~~546	$	0.06
Issued	—	—
Vested	—	—
Repurchased	—	—
Outstanding at March 31, 2025	3,472,543	$	0.06
Outstanding at June 30, 2025	3,472,546	$	0.06

Stock-Based Compensation

The following table below summarizes the classification of the Company’s stock-based compensation expense related to stock options and restricted common stock awards in the condensed consolidated statements of operations and comprehensive loss:

			Three Months EndedMarch 31,
	Three Months EndedJune 30,				Six Months EndedJune 30,
(in thousands)	2025		2024		2025		2024
Stock-based compensation expense:
Research and development	$	3,218	$	4,221	$	26,~~856~~074	$	26,~~725~~946
General and administrative	3,107		3,870		25,~~825~~932		26,~~484~~354
Total stock-based compensation expense	$	6,325	$	8,091	$	512,~~681~~006	$	513,~~209~~300

9.Significant Agreements

The Company’s significant agreements are disclosed in Note 9, License and Collaboration Agreements, in the audited consolidated financial statements for the year ended December 31, 2024, and notes thereto, included in the Company’s Annual Report on Form 10-K that was filed with the SEC on February 28, 2025. Since the date of those financial statements, there have been no changes to the Company’s significant agreements except those discussed below.

financial statements, there have been no changes to the Company’s significant agreements except those discussed below.

Bristol-Myers Squibb — Related Party

Supplemental information related to the BMS Collaboration Agreement consisted of the following:

(in thousands)	~~March~~June 3130,2025		December 31,2024
Collaboration receivable — related party	$	17120	$	—
Deferred revenue — related party	$	8,~~214~~315	$	7,092
Deferred revenue, net of current — related party	$	6059,~~658~~562	$	63,218

			Three Months Ended March 31,
	Three Months Ended June 30,				Six Months Ended June 30,
(in thousands)	2025		2024		2025		2024
Revenue recognized that was included in contract liability at the beginning of the period	$	1,061	$	—	$	12,~~419~~502	$	—
Revenue recognized from performance obligations fully or partially satisfied in previous periods	$	5	$	—	$	—3	$	—

As of ~~March~~June 3130, 2025, the aggregate amount of the transaction price allocated to performance obligations under the BMS Collaboration Agreement that are partially unsatisfied was $7067.39 million. The Company recognizes the portion of the transaction price as the single performance obligation is satisfied, using an input method, in proportion to costs incurred to date as compared to total costs incurred and expected to be incurred in the future to satisfy the underlying obligation.

Cystic Fibrosis Foundation

In July 2025, the Company entered into an agreement with the Cystic Fibrosis Foundation (“CFF”), under which CFF has agreed to provide up to $24 million in additional funding to accelerate the development of Prime Editors designed to permanently correct cystic fibrosis-related lung disease. The $24 million funding will be provided in two equal tranches, subject to certain closing conditions and scientific milestones. The first tranche includes a $6 million equity investment in the Company, which was received as part of the August 2025 offering.

CFF’s additional investment builds on initial funding received under the Company’s therapeutic development agreement with CFF in January 2024.

Beam Collaboration Agreement — Related Party

The Company is currently engaged in arbitration proceedings with Beam Therapeutics, Inc. (“Beam”) regarding the parties’ collaboration and license agreement (the “Agreement”). A dispute arose between the parties following the Company’s March 18, 2025 announcement that it is developing a Prime Editing-based treatment for alpha-1 antitrypsin deficiency (“AATD”). On April 16, 2025, Beam filed an arbitration demand with the American Arbitration Association (“AAA”), alleging that the Company has breached the Agreement by developing a product for the treatment of AATD and by allegedly not complying with certain obligations to transfer technical information to Beam pursuant to the Agreement. Beam also makes related claims for trade secret misappropriation and various business torts based on similar allegations, including allegations made on information and belief. Beam seeks both declaratory, injunctive, and monetary relief, but has not yet quantified the amount of damages it seeks. On April 18, 2025, the Company filed an arbitration demand with the AAA seeking a declaration that the Company’s AATD program is within our “Field” as defined by the Agreement. The arbitrations have been consolidated, and the consolidated proceeding remains in its early stages. If the final resolution of the matter is adverse to the Company, the arbitration panel may provide Beam with relief including, among other things, monetary damages and/or an order that the Company cease work on its AATD program and transfer such program to Beam.

10.Net Loss per Share

Basic and diluted net loss per common share attributable to common stockholders was calculated as follows:

			Three Months EndedMarch 31,
	Three Months EndedJune 30,				Six Months EndedJune 30,
(in thousands, except share and per share amounts)	2025		2024		2025		2024
Numerator:
Net loss attributable to common stockholders	$	(52,591)	$	(55,327)	$	(51104,~~890~~481)	$	(45101,~~761~~088)
Denominator:
Weighted-average common shares outstanding, basic and diluted	129,185,918		119,188,866		~~130~~128,~~884~~439,~~490~~349		~~104~~111,~~466~~827,~~178~~522
Net loss per share attributable to common stockholders, basic and diluted	$	(0.41)	$	(0.46)	$	(0.4081)	$	(0.4490)

The weighted-average number of common shares outstanding used in the basic and diluted net loss per share calculations includes the weighted-average effect of pre-funded warrants sold by the Company to purchase 3,200,005 shares of the Company's common stock. The shares of common stock underlying the pre-funded warrants are considered outstanding for the purposes of computing earnings per share, because the shares may be issued for little or no consideration, they are fully vested, and the pre-funded warrants are immediately exercisable upon their issuance date.

Diluted net loss per share available to common stockholders is computed by giving effect to all potentially dilutive common stock equivalents outstanding for the period. For purposes of this calculation, preferred stock, unvested restricted stock and stock options to purchase common stock were considered common stock equivalents but had been excluded from the calculation of diluted net loss per share available to common stockholders as their effect was anti-dilutive. In periods in which the Company reports a net loss available to common stockholders, diluted net loss per share available to common stockholders is the same as basic net loss per share available to common stockholders, since dilutive common shares are not assumed to have been issued if their effect is anti-dilutive.

The following common stock equivalents were excluded from the calculation of diluted net loss per share applicable to common stockholders for the periods indicated because including them would have had an anti-dilutive effect:

As of March 31,

As of June 30,

2025

2024

Anti-dilutive common stock equivalents:

Options to purchase common stock

1517,~~919~~685,~~190~~319

1112,~~522~~007,~~606~~443

Unvested restricted common stock

3,472,~~543~~546

43,~~406~~729,~~163~~877

Total anti-dilutive common stock equivalents:

1921,~~391~~157,~~733~~865

15,~~928~~737,~~769~~320

MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS

You should read the following discussion and analysis of our financial condition and results of operations together with our condensed consolidated financial statements and the related notes thereto included elsewhere in this Quarterly Report on Form 10-Q and our audited consolidated financial statements and notes and Management’s Discussion and Analysis of Financial Condition and Results of Operations, included in our Annual Report on Form 10-K for the year ended December 31, 2024, filed with the SEC on February 28, 2025. As discussed in the section titled “Special Note Regarding Forward-Looking Statements,” the following discussion and analysis contains forward-looking statements that involve risks and uncertainties, such as statements regarding our plans, objectives, expectations, intentions, or projections, as well as assumptions that, if they never materialize or prove incorrect, could cause our results to differ materially from those discussed in these forward-looking statements. Factors that could cause or contribute to such differences include, but are not limited to, those discussed in the “Risk Factors” section of our Annual Report on Form 10-K for the year ended December 31, 2024. Our historical results are not necessarily indicative of the results that may be expected for any period in the future.

Overview

We are a biotechnology company committed to delivering a new class of differentiated one-time curative genetic therapies. We are deploying Prime Editing technology, which we believe is a versatile, precise, and efficient gene editing technology.

InGoing ~~March~~forward, ~~2025~~we will focus our resources on advancing our in vivo liver franchise, where we ~~announced~~are aadvancing ~~preclinical~~programs ~~program~~to ~~for~~cure two of the ~~treatment~~largest ofgenetic ~~alpha~~liver diseases, Wilson’s Disease and Alpha-1 ~~antitrypsin~~Antitrypsin ~~deficiency~~Deficiency, ~~our~~or ~~second~~AATD. ~~program~~Both ~~within~~programs ~~our~~are ~~liver~~currently ~~franchise,~~in ~~which,~~late ~~with~~stages ~~our~~of pre-clinical development and are on track for IND and/or CTA filings in the first half of 2026 for Wilson’s Disease ~~program,~~and wethe ~~expect~~middle toof ~~advance~~2026 ~~into~~for AATD. We intend to leverage the ~~clinic~~modularity ~~next~~of ~~year~~our platform to expeditiously and efficiently develop these programs supported by our universal liver lipid nanoparticle along with potential regulatory, clinical and other synergies from our modular technology.

~~Chronic~~We ~~granulomatous~~will ~~disease~~also iscontinue our ~~most~~in ~~advanced~~vivo ~~blood~~Cystic Fibrosis program with support from the Cystic Fibrosis Foundation, and weour ~~are~~efforts ~~advancing~~to ~~PM359~~develop asPrime ~~our~~Edited ~~candidate~~CAR-T products for hematology, immunology and oncology in partnership with Bristol-Myers Squibb Company. In addition, we will continue to pursue additional business development opportunities to accelerate innovation, ensure the ~~treatment~~broadest application of ~~this~~Prime ~~disease~~Editing, and further bolster our financial resources.

WeFor ~~believe~~example, ~~our~~in July 2025, we announced that the Cystic Fibrosis Foundation has agreed to provide us with up to $24 million in additional funding to accelerate the development of Prime ~~Editing~~Editors ~~programs~~designed ~~are~~to ~~well~~permanently correct cystic fibrosis-~~positioned~~related tolung ~~leverage~~disease. The Cystic Fibrosis Foundation’s additional investment builds on initial funding received under our therapeutic development agreement with the ~~clinical,~~Cystic ~~regulatory~~Fibrosis Foundation in January 2024, and ~~manufacturing~~reflects ~~advancements~~its ~~made~~interest in Prime Editing as a potentially curative approach for cystic fibrosis. We intend to ~~date~~leverage ~~across~~the ~~gene~~technology’s ~~therapy~~versatility and modularity to address multiple disease-causing mutations, ~~gene~~potentially ~~editing~~treating the vast majority of people with cystic fibrosis. We will initially focus on a program targeting G542X, one of the most prevalent cystic fibrosis-causing nonsense mutations and ~~delivery~~one ~~modalities~~for towhich ~~accelerate~~there ~~progression~~are no available therapies. In addition, we will continue to ~~clinical~~advance ~~trials~~hotspot and ~~potential~~PASSIGE-based ~~approval~~approaches for other mutations with funding received from the Cystic Fibrosis Foundation under its initial commitment in 2024.

We recently announced initial data from the first patient dosed in our Phase 1/2 trial in chronic granulomatous disease, or CGD. In this patient dosed, a single dose of PM359, our candidate for the treatment of CGD, led to 58% dihydrorhodamine positivity by Day 15, 66% by Day 30 and 71% by Day 60, exceeding levels of dihydrohomadine positivity believed to be potentially curative. In the second patient dosed in the trial, a single dose of PM359 led to a 70% dihydrorhodamine positivity by day 15 and 80% by day 30. In both patients, we also observed successful manufacturing from a single mobilization, no serious adverse events related to PM359 and rapid neutrophil and platelet engraftment when compared to other commercially available autologous genetic therapy benchmarks. We continue to believe PM359 has the potential to transform the care of p47phox CGD. We are planning to have regulatory interactions based on the current data set.

Components of Our Results of Operations

Revenues

To date, we have not generated any revenue from product sales and do not expect to generate any revenue from the sale of products for the foreseeable future. Our revenues to date have been generated through research collaboration and license agreements. We recognize revenue over the expected performance period under each agreement. We expect that our revenue for the next several years will be derived primarily from our current collaboration agreements and any additional collaborations that we may enter into in the future. To date, we have not received any royalties under any of our existing collaboration agreements.

Research and Development Expenses

Research and development expenses consist primarily of costs incurred in connection with the development and research of our immediate target indications and our differentiation target indications. These expenses include:

•personnel-related expenses, including salaries, bonuses, benefits and stock-based compensation for employees engaged in manufacturing, research and development functions;

•expenses incurred in connection with continuing our current research programs and preclinical and clinical development of any product candidates we may identify, including under agreements with third parties, such as consultants and contractors;

•the cost of developing and validating our manufacturing process for use in our preclinical and clinical studies;

•laboratory supplies and research materials;

•facilities, depreciation and other expenses related to research and development activities, which include direct or allocated expenses for rent and maintenance of facilities, and utilities;

•the cost allocated to acquire in-process research and development, with no alternative future use associated with asset acquisitions or transactions to license intellectual property, such as our Broad License Agreement; and

•expenses incurred in connection with our Pledge to Broad Institute.

We expense all research and development costs in the periods in which they are incurred. Most of our research and development expenses have been related to early stage development activities. In the future, external research and development costs for any individual product candidate will be tracked commencing upon product candidate nomination. We do not allocate employee costs, costs associated with our discovery efforts, laboratory supplies, and facilities expenses, including depreciation or other indirect costs, to specific product development programs because these costs are deployed across multiple programs and our platform and, as such, are not separately classified.

Upfront and milestone payments made are accrued for and expensed when the achievement of the milestone is probable up to the point of regulatory approval. Milestone payments made upon regulatory approval will be capitalized and amortized over the remaining useful life of the related product.

We expect our research and development expenses to continue to increase substantially for the foreseeable future with our planned research and development activities related to developing any future product candidates, including investments in manufacturing, as we advance any product candidates we may identify and begin to conduct clinical trials, and with our obligations under the BMS Collaboration Agreement.

General and Administrative Expenses

General and administrative expenses consist of salaries and personnel-related costs, including stock-based compensation, for our personnel in executive, legal, finance and accounting, human resources and other administrative functions. General and administrative expenses also include legal fees relating to patents and corporate matters; professional fees paid for accounting, auditing, consulting and tax service; insurance costs; office and information technology costs; and facilities, depreciation and other general and administrative expenses, which include direct or allocated expenses for rent and maintenance of facilities and utilities.

corporate matters; professional fees paid for accounting, auditing, consulting and tax service; insurance costs; office and information technology costs; and facilities, depreciation and other general and administrative expenses, which include direct or allocated expenses for rent and maintenance of facilities and utilities.

We anticipate that our general and administrative expenses will increase in the future as we increase our headcount to support research and development activities; increased accounting, legal, insurance, and investor and public relations costs as we continue to operate as a public company; and additional intellectual property-related expenses as we file patent applications to protect innovations arising from our research and development activities.

Other Income (Expense)

Other income (expense), net primarily consists of interest and other income earned on our short-term investments and the change in the fair value of our short-term investment in Beam Therapeutics Inc. (“Beam”), a related party, in connection with the Beam Collaboration Agreement, which is discussed in greater detail in Note 9, License and Collaboration Agreements, to the audited consolidated financial statements in our Annual Report on Form 10-K for the year ended December 31, 2024.

Results of Operations

Comparison of the Three Months Ended ~~March~~June 3130, 2025 and 2024

Operating Expenses

Research and Development Expenses

	Three Months EndedMarch 31,
	Three Months EndedJune 30,
(in thousands)	2025		2024		Change
Research and development expenses:
Personnel expenses	$	14,~~800~~448	$	1516,~~007~~198	$	(~~207~~1,750)
Research costs	11,036		11,619		(583)
Facility related	13,765		11,302		2,463
Facility related	10,969		7,334		3,635
Research costs	8,116		11,390		(3,274)
License, intellectual property fees, and other	12,~~425~~812		1,~~675~~250		~~(250)~~1,562
Clinical expense	1,173		547		626
Professional and consultant fees	1,660		1,281		379
Professional and consultant fees	1,159		1,592		(433)
Clinical expense	574		1,650		(1,076)
Total research and development expenses	$	4041,~~562~~375	$	3743,~~774~~071	$	2(1,~~788~~696)

The $21.87 million ~~increase~~decrease in research and development expense for the three months ended ~~March 31~~June 30, 2025 as compared to the three months ended ~~March 31~~June 30, 2024 was primarily driven by ~~a $3.6 million increase in facility-related expense primarily due to the expansion and build out of our laboratory space at 60 First Street and 500 Arsenal Street.~~:

•$3.3 million decrease in research costs due to the deprioritization of our CGD programs as we strategically focus our internal efforts on advancing our in vivo liver franchise;

•$1.8 million decrease in personnel expenses, driven primarily by a decrease of $1.0 million in stock-based compensation expense and fewer R&D personnel resulting from workforce reduction; and

•$1.1 million decrease in clinical expenses due to the deprioritization of our CGD programs and discontinuation of our efforts to independently advance our PM359 program to focus on our liver franchise and programs funded through external partnerships.

These were offset by:

•$2.5 million increase in facility-related expense primarily due to the expansion and build out of our laboratory space at 60 First Street and 500 Arsenal Street; and

•$1.6 million increase in license and IP costs as we advance our in vivo liver franchise and pipeline.

General and Administrative Expenses

Three Months EndedMarch 31,

Three Months EndedJune 30,

(in thousands)

2025

2024

Change

General and administrative expenses:

Personnel expenses

7,~~156~~082

56,~~884~~956

~~1,272~~126

Professional and consultant fees

3,~~272~~956

32,~~110~~821

~~162~~1,135

Facility related and other

2,~~856~~079

2,~~164~~824

~~692~~(745)

Total general and administrative expenses

13,~~284~~117

1112,~~158~~601

~~2,126~~516

The $20.15 million increase in general and administrative expense for the three months ended ~~March 31~~June 30, 2025 as compared to the three months ended ~~March 31~~June 30, 2024 is primarily driven by a $1.31 million increase in ~~personnel~~professional and consultant fees due to an increase in corporate legal expenses.

Other Income (Expense)

Three Months EndedMarch 31,

Three Months EndedJune 30,

(in thousands)

2025

2024

Change

Other income:

Interest income

~~1,182~~743

~~676~~611

~~506~~132

Accretion (amortization) of investments

~~339~~530

~~830~~1,486

(~~491~~956)

Change in fair value of short-term investment — related party

(~~1,056~~505)

(1,~~166~~925)

(21,~~222)~~420

Other income, net

3718

4239

(521)

Total other income, net

~~502~~786

~~2,714~~211

~~(2,212)~~575

Change in Fair Value of Related Party Short-Term Investment

The change in fair value of related party short-term investment for each of the periods presented is a result of Beam’s stock price movement.

~~Liquidity~~Comparison ~~and~~of ~~Capital~~the ~~Resources~~Six Months Ended June 30, 2025 and 2024

~~Since~~Operating our inception, we have incurred significant operating losses. We expect to incur significant expenses and operating losses for the foreseeable future as we commence the clinical development of our programs and continue our platform development and early-stage research activities. We have not yet commercialized any products and we do not expect to generate revenue from sales of products for several years, if at all. To date, we have funded our operations primarily with proceeds from sales of preferred stock and from our public offerings and through payments from our collaboration partners. As of March 31, 2025, we had cash, cash equivalents, and investments of $144.3 million, excluding our restricted cash, or $158.3 million, including restricted cash.Expenses

~~Going~~Research ~~Concern~~and Development Expenses

Since our inception, we have incurred substantial losses. As of March 31, 2025, we had an accumulated deficit of $739.1 million and we expect to generate operating losses and negative operating cash flows for the foreseeable future. As stated above, as of March 31, 2025, we maintained cash, cash equivalents, short-term investments, and related party short-term investments of $144.3 million.


	Six Months Ended June 30,
(in thousands)	2025		2024		Change
Research and development expenses:
Personnel expenses	$	29,248	$	31,205	$	(1,957)
Facility related	24,734		18,636		6,098
Research costs	19,152		23,009		(3,857)
License, intellectual property fees, and other	4,237		2,925		1,312
Professional and consultant fees	2,819		2,873		(54)
Clinical expense	1,747		2,197		(450)
Total research and development expenses	$	81,937	$	80,845	$	1,092

In accordance with Accounting Standards Codification, or ASC, 205-40, Going Concern, or ASC 205-40, we evaluated whether there are conditions and events, considered in the aggregate, that raise substantial doubt about our ability to continue as a going concern within one year after the date on which this Quarterly Report on Form 10-Q is filed. Based on the our cash, cash equivalents, short-term investments, and related party short-term investments as of March 31, 2025, our current and forecasted level of operations and forecasted cash flows we determined that substantial doubt exists about our ability to continue as a going concern for 12 months from the date these condensed consolidated financial statements are issued. Our ability to continue as a going concern is dependent upon our ability to obtain the necessary financing to meet our obligations and repay our liabilities arising from normal business operations when they come due. Management plans to provide for capital requirements through financing or other transactions, and selling shares under our “at the market offering” program. There can be no assurance that we will be able to raise additional capital to fund operations with terms acceptable to us, or at all. Because certain elements of our plans to mitigate the conditions that raised substantial doubt about our ability to continue as a going concern are outside of our control, including the ability to raise capital through an equity or other financing, those elements cannot be considered probable according to ASC 205-40, and therefore cannot be considered in the evaluation of mitigating factors.

The condensed consolidated financial statements as of March 31, 2025 have been prepared under the assumption that we will continue as a going concern for the next 12 months and that contemplates the realization of assets and satisfaction of liabilities and commitments in the normal course of business. Our ability to continue as a going concern is dependent upon our uncertain ability to obtain additional capital, reduce expenditures and/or execute on its business plan. These condensed consolidated financial statements do not include any adjustments that might result from the outcome of this uncertainty.

The $1.1 million increase in research and development expense for the six months ended June 30, 2025 as compared to the six months ended June 30, 2024 was primarily driven by:

•$6.1 million increase in facility-related expense primarily due to the expansion and build out of our laboratory space at 60 First Street and 500 Arsenal Street; and

•$1.3 million increase in license and IP costs as we advance our in vivo liver franchise and pipeline.

This is offset by:

•$3.9 million decrease in research costs due to the deprioritization of our CGD programs as we strategically focus our internal efforts on advancing our in vivo liver franchise; and

•$2.0 million decrease in personnel expenses, driven primarily by a decrease of $0.9 million in stock-based compensation expense and fewer R&D personnel resulting from the workforce reduction.

General and Administrative Expenses


	Six Months Ended June 30,
(in thousands)	2025		2024		Change
General and administrative expenses:
Personnel expenses	$	14,238	$	12,840	$	1,398
Professional and consultant fees	7,228		5,931		1,297
Facility related and other	4,935		4,988		(53)
Total general and administrative expenses	$	26,401	$	23,759	$	2,642

The $2.6 million increase in general and administrative expense for the six months ended June 30, 2025 as compared to the six months ended June 30, 2024 is primarily driven by:

•$1.4 million increase in personnel expenses in connection with the reduction in force, primarily consisting of one-time severance payments and other employee termination-related expenses; and

•$1.3 million increase in professional and consultant fees due an increase in corporate legal expenses.

Other Income (Expense)


	Six Months Ended June 30,
(in thousands)	2025		2024		Change
Other income:


Interest income	$	1,925	$	1,287	$	638
Accretion (amortization) of investments	869		2,316		(1,447)
Change in fair value of short-term investment — related party	(1,561)		(759)		(802)
Other income, net	55		81		(26)
Total other income, net	$	1,288	$	2,925	$	(1,637)

Accretion (amortization of investments

Accretion (amortization) of investments for each of the periods presented is a result of increase (decrease) in the value of the our marketable securities purchased at a discount (premium) to their face value.

Liquidity and Capital Resources

Since our inception, we have incurred significant operating losses. We expect to incur significant expenses and operating losses for the foreseeable future as we advance the preclinical development of our current research programs, commence the clinical development of any product candidates we may identify, and continue our platform development and early-stage research activities. We have not yet commercialized any products and we do not expect to generate revenue from sales of products for several years, if at all. To date, we have funded our operations primarily with proceeds from sales of preferred stock and from our public offerings and through payments from our collaboration partners. As of June 30, 2025, we had cash, cash equivalents, and investments of $101.8 million, excluding our restricted cash, or $115.4 million, including restricted cash.

In November 2023, we entered into an Open Market Sale AgreementSM (the “Sales Agreement”) with Jefferies LLC (“Jefferies”) under which we may, from time to time, issue and sell shares of our common stock having aggregate sales proceeds of up to $300.0 million, in a series of one or more at-the-market equity offerings (the “ATM Program”). Effective July 30, 2025, we terminated the sales agreement prospectus (the “ATM Prospectus”) filed with the shelf registration statement on Form S-3 (File No. 333-275321) and related to the shares of our common stock issuable pursuant to the Sales Agreement. As a result, we will not make any sales of our common stock pursuant to the Sales Agreement, unless and until a new prospectus, prospectus supplement or a new registration statement is filed. Other than the termination of the ATM Prospectus, the Sales Agreement remains in full force and effect. As of the date of this Quarterly Report on Form 10-Q, we have not sold any shares of common stock under the 2023 ATM program.

In August 2025, we issued and sold 43,700,000 shares of our common stock, including 5,700,000 shares pursuant to the exercise of the underwriters’ option to purchase additional shares, at a price to the public of $3.30 per share. As a result of the offering, we received approximately $138.2 million in net proceeds, after deducting underwriting discounts, commissions and offering costs of approximately $6.0 million.

Cash Flows

The following table summarizes our sources and uses of cash for each of the periods presented:

Three Months EndedMarch 31,

Six Months EndedJune 30,

(in thousands)

2025

2024

Net change in cash, cash equivalents and restricted cash:

Net cash used in operating activities

(4890,~~857~~269)

(67113,~~707~~198)

Net cash used in investing activities

(4744,~~742~~971)

(3730,~~032~~138)

Net cash provided by financing activities

6,~~000~~197

157,~~327~~362

Net change in cash, cash equivalents, and restricted cash

(90129,~~599~~043)

5214,~~588~~026

Operating Activities

Net cash used in operating activities for the ~~three~~six months ended ~~March~~June 3130, 2025 was driven primarily by the following uses of cash:

•$51104.95 million net loss;

•$62.74 million change in ~~accrued~~deferred ~~expenses~~revenue ~~and~~— ~~other~~related ~~current liabilities~~party; ~~and~~

•$12.41 million change in ~~deferred~~lease ~~revenue — related party.~~liabilities

~~These~~•$1.7 ~~were~~million ~~offset~~change ~~by:~~in accrued expenses and other current liabilities; and

•$101.75 million ~~of non-cash amounts included in net loss, which primarily consisted of stock-based compensation expense, non-cash lease expense, depreciation expense, and~~ change in ~~fair~~accounts ~~value of short-term investment — related party; and~~payable.

~~•$1.9 million change in accounts payable.~~29

~~Net~~These were offset by $21.3 million of non-cash ~~used~~amounts included in ~~operating~~net ~~activities~~loss, ~~for~~which ~~the~~primarily ~~three~~consisted ~~months~~of ~~ended~~stock-based ~~March 31~~compensation expense, ~~2024~~non-cash ~~was~~lease ~~driven~~expense, ~~primarily~~depreciation byexpense, ~~the~~and ~~following~~change ~~uses~~in fair value of ~~cash:~~short-term investment — related party.

~~•$45.8~~Net ~~million~~cash ~~net~~used ~~loss;~~in operating activities for the six months ended June 30, 2024 was driven primarily by the following uses of cash:

•$101.1 million net loss;

•$13.5 million change in accrued settlement payment — related party;

•$5.9.2 million change in accounts payable;

•$4.5.7 million change in ~~accrued~~lease ~~expenses and other current~~ liabilities;

•$3.59 million change in ~~lease~~accrued expenses and other current liabilities; and

•$2.85 million change in prepaid and other current assets.

These were offset by $822.28 million of non-cash amounts included in net loss, which primarily consisted of stock-based compensation expense, non-cash lease expense, and depreciation expense.

Investing Activities

Net cash used in investing activities for the ~~three~~six months ended ~~March~~June 3130, 2025 was driven primarily by the following:

•$4541.30 million of purchases of marketable securities, net of maturities; and

•$2.4.0 million of purchases of property and equipment.

28Net cash used in investing activities for the six months ended June 30, 2024 was driven primarily by the following:

~~Net~~•$25.9 million ~~cash~~of ~~used~~purchases inof ~~investing~~marketable ~~activities for the three months ended March 31~~securities, ~~2024~~net ~~was~~of ~~driven~~maturities; ~~primarily by the following:~~and

•$344.7 2 million of purchases of ~~marketable~~property ~~securities, net of maturities;~~ and equipment.

•$2.3 million of purchases of property and equipment.

Financing Activities

Net cash provided by financing activities for the ~~three~~six months ended ~~March~~June 3130, 2025 was driven by $6.0 million of proceeds received under our agreement with Cystic Fibrosis Foundation.

Net cash provided by financing activities for the ~~three~~six months ended ~~March~~June 3130, 2024 was driven primarily by the following:

•$132.1 million of proceeds from issuances of common stock in ~~the~~ our February 2024 public offering;

•$18.8 million of proceeds from issuance of pre-funded warrants contemporaneous with our February 2024 public offering; and

•$6.0 million of proceeds received under our agreement with Cystic Fibrosis Foundation.

Funding Requirements

To date, we have not generated any revenue from product sales. We do not expect to generate revenue from product sales unless and until we successfully complete preclinical and clinical development of, receive regulatory approval for, and commercialize a product candidate and we do not know when, or if at all, that will occur. We expect our expenses to increase in connection with our ongoing activities, particularly as we advance the preclinical activities and studies and initiate clinical trials. In addition, if we obtain regulatory approval for any product candidates, we expect to incur significant expenses related to product sales, marketing, and distribution to the extent that such sales, marketing and distribution are not the responsibility of potential collaborators. Further, we have incurred, and expect to continue to incur, costs associated with operating as a public company. The timing and amount of our operating expenditures will depend largely on the factors set out above. For more information, refer to the section titled “Risk Factors” in our Annual Report on Form 10-K for the year ended December 31, 2024, and the “Risk Factors” section of subsequent Quarterly Reports on Form 10-Q.

We believe our existing cash, cash equivalents, and investments, will be sufficient to fund our operating expenses and capital expenditure requirements into the first half of 2026. We have based this estimate on assumptions that may prove to be wrong, and we could exhaust our available capital resources sooner than we expect. We expect that we will require additional funding to:30

marketing and distribution are not the responsibility of potential collaborators. Further, we have incurred, and expect to continue to incur, costs associated with operating as a public company. The timing and amount of our operating expenditures will depend largely on the factors set out above. For more information, refer to the section titled “Risk Factors” in our Annual Report on Form 10-K for the year ended December 31, 2024, and the “Risk Factors” section of subsequent Quarterly Reports on Form 10-Q.

We believe our existing cash, cash equivalents, and investments, including funds from our August 2025 offering, will be sufficient to fund our operating expenses and capital expenditure requirements into 2027. We have based this estimate on assumptions that may prove to be wrong, and we could exhaust our available capital resources sooner than we expect. We expect that we will require additional funding to:

•continue our current research development activities;

•identify product candidates;

•evaluate strategic alternatives and potential partnership opportunities for PM359, including our ability to execute and realize the anticipated benefits of any strategic alternatives we may pursue;

•develop, maintain, expand and protect our intellectual property portfolio and defend intellectual property-related claims;

•maintain existing collaborations or strategic relationships and identify and enter into future license agreements and collaborations with third parties;

•initiate preclinical testing and clinical trials for our future product candidates we identify;

•develop, maintain, expand and protect our intellectual property portfolio;

•further develop our Prime Editing platform; and

•hire additional ~~research,~~personnel ~~clinical~~to ~~and~~support ~~scientific~~our ~~personnel~~strategic priorities.

If we receive regulatory approval for any of our product candidates, we expect to incur significant commercialization expenses related to product manufacturing, sales, marketing and distribution, depending on where we choose to commercialize ourselves.

29Until such time, if ever, as we can generate substantial product revenue, we expect to finance our cash needs through a combination of private and public equity offerings, debt financings, additional collaborations, strategic alliances, and marketing, distribution or licensing arrangements with third parties. To the extent that we raise additional capital through the sale of equity or convertible debt securities, ownership interest may be materially diluted, and the terms of such securities could include liquidation or other preferences that adversely affect your rights as a common stockholder. Debt and equity financing, if available, may involve agreements that include restrictive covenants that limit our ability to take specified actions, such as incurring additional debt, making capital expenditures or declaring dividends. If we raise funds through collaborations, strategic alliances or marketing, or distribution or licensing arrangements with third parties, we may have to relinquish valuable rights to our technologies, any future revenue streams, research programs or product candidates or grant licenses on terms that may not be favorable to us. If we are unable to raise additional funds through equity or debt financings or other arrangements when needed, we may be required to delay, reduce or eliminate our product development or future commercialization efforts, or grant rights to develop and market product candidates that we would otherwise prefer to develop and market ourselves.

Until such time, if ever, as we can generate substantial product revenue, we expect to finance our cash needs through a combination of private and public equity offerings, debt financings, additional collaborations, strategic alliances, and marketing, distribution or licensing arrangements with third parties. To the extent that we raise additional capital through the sale of equity or convertible debt securities, ownership interest may be materially diluted, and the terms of such securities could include liquidation or other preferences that adversely affect your rights as a common stockholder. Debt financing and preferred equity financing, if available, may involve agreements that include restrictive covenants that limit our ability to take specified actions, such as incurring additional debt, making capital expenditures or declaring dividends. If we raise funds through collaborations, strategic alliances or marketing, or distribution or licensing arrangements with third parties, we may have to relinquish valuable rights to our technologies, any future revenue streams, research programs or product candidates or grant licenses on terms that may not be favorable to us. If we are unable to raise additional funds through equity or debt financings or other arrangements when needed, we may be required to delay, reduce or eliminate our product development or future commercialization efforts, or grant rights to develop and market product candidates that we would otherwise prefer to develop and market ourselves.

Contractual Obligations and Other Commitments

We enter into contracts in the normal course of business with contract organizations and other vendors to assist in the performance of our research and development activities, and other services and products for operating purposes. These contracts generally provide for termination on notice, and therefore are cancellable contracts and not included in the table of contractual obligations and commitments.

During the ~~three~~six months ended ~~March~~June 3130, 2025, except for the minimum lease commitments disclosed in Note 6, Leases, to the unaudited condensed consolidated financial statements in this Quarterly Report on Form 10-Q, there were no significant changes to our contractual obligations and commitments described under Management’s Discussion and Analysis of Financial Condition and Results of Operations in our Annual Report on Form 10-K for the year ended December 31, 2024.

were no significant changes to our contractual obligations and commitments described under Management’s Discussion and Analysis of Financial Condition and Results of Operations in our Annual Report on Form 10-K for the year ended December 31, 2024.

Critical Accounting Policies and Significant Judgments and Estimates

Our management’s discussion and analysis of our financial condition and results of operations is based on our condensed consolidated financial statements, which have been prepared in accordance with U.S. GAAP. The preparation of these condensed consolidated financial statements requires us to make estimates and assumptions that affect the reported amounts of assets and liabilities, the disclosures of contingent assets and liabilities at the date of the condensed consolidated financial statements and the reported amounts of revenues and expenses incurred during the reporting periods. We base our estimates on historical experience, known trends and events, and various other factors that we believe are reasonable under the circumstances, the results of which form the basis for making judgments about the carrying values of assets and liabilities recorded revenues and expenses that are not readily apparent from other sources. We evaluate our estimates and assumptions on an ongoing basis. Actual results may differ from these estimates.

During the ~~three~~six months ended ~~March~~June 3130, 2025, there were no material changes to our critical accounting policies and significant judgements described under Management’s Discussion and Analysis of Critical Accounting Policies and Significant Judgments and Estimates which are included in our Annual Report on Form 10-K for the year ended December 31, 2024.

Recently Issued and Adopted Accounting Pronouncements

A description of recently issued accounting pronouncements that may potentially impact our financial position and results of operations is disclosed in Note 2, Summary of Significant Accounting Policies, to our audited financial statements for the year ended December 31, 2024, and notes thereto, included in our Annual Report on Form 10-K and in this Quarterly Report on Form 10-Q.

Emerging Growth Company Status

The Jumpstart Our Business Startups Act of 2012 permits an “emerging growth company” such as us to take advantage of an extended transition period to comply with new or revised accounting standards applicable to public companies until those standards would otherwise apply to private companies. We have elected not to “opt out” of such extended transition period, which means that when a standard is issued or revised and it has different application dates for public or private companies, we will adopt the new or revised standard at the time private companies adopt the new or revised standard and will do so until such time that we either (i) irrevocably elect to “opt out” of such extended transition period or (ii) no longer qualify as an emerging growth company. As a result of this election, our condensed consolidated financial statements may not be comparable to other public companies that comply with new or revised accounting pronouncements as of public company effective dates. We may choose to early adopt any new or revised accounting standards whenever such early adoption is permitted for private companies.

Item 3. Quantitative and Qualitative Disclosures About Market Risk

Interest Rate Risk

We are exposed to market risk related to changes in interest rates of our investment portfolio of cash equivalents and investments. As of ~~March~~June 3130, 2025, we held cash, cash equivalents, investments, and restricted cash of $~~158~~115.34 million, which consisted of cash, money market funds, equity securities, and debt securities. Our primary exposure to market risk is interest income sensitivity, which is affected by changes in the general level of U.S. interest rates. The fair value of our cash equivalents, consisted of our money market funds, and investments are subject to change as a result of potential changes in market interest rates. Due to the short-term maturities of our cash equivalents and investments and the low risk profile of our investments, an immediate 10 percent change in interest rates would not have a material effect on the fair market value of our cash equivalents or investments.

Effects of Inflation

Inflation generally affects us by increasing our cost of labor and research and development costs. Although we do not believe that inflation has had a material impact on our financial position or results of operations to date, we may experience some effect in the near future (especially if inflation rates continue to rise) due to an impact on the costs to conduct research and development, labor costs we incur to attract and retain qualified personnel, and other operational costs. Inflationary costs could adversely affect our business, financial condition and results of operations.

Item 4. Controls and Procedures

We have established disclosure controls and procedures (as defined in Rules 13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934, as amended (the “Exchange Act”)), designed to ensure that information required to be disclosed in the reports that we file or submit under the Exchange Act is recorded, processed, summarized and reported within the time periods specified in the SEC’s rules and forms and is accumulated and communicated to management, including ~~the~~our ~~principal~~Chief ~~executive~~Executive ~~officer~~Officer (~~our~~who ~~Chief~~serves ~~Executive~~as ~~Officer)~~both ~~and~~our principal ~~financial~~executive officer ~~(our~~and ~~Chief~~principal ~~Financial~~financial ~~Officer~~officer), to allow timely decisions regarding required disclosure. In addition, the design of disclosure controls and procedures must reflect the fact that there are resource constraints and that management is required to apply judgment in evaluating the benefits of possible controls and procedures relative to their costs.

Internal Control over Financial Reporting

Disclosure Controls and Procedures

Our management, with the participation of our Chief Executive Officer (who serves as both our principal executive officer and principal financial officer), evaluated, as of the end of the period covered by this Quarterly Report on Form 10-Q, the effectiveness of our disclosure controls and procedures (as defined in Rules 13a-15(e) and 15d-15(e) under the Exchange Act). Based on that evaluation, our Chief Executive Officer concluded that our disclosure controls and procedures were effective as of June 30, 2025. Management recognizes that any controls and procedures, no matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives and management necessarily applies its judgment in evaluating the cost-benefit relationship of possible controls and procedures. Our disclosure controls and procedures have been designed to provide reasonable assurance of achieving their objectives.

the cost-benefit relationship of possible controls and procedures. Our disclosure controls and procedures have been designed to provide reasonable assurance of achieving their objectives.

Changes in Internal Control Over Financial Reporting

There were no changes in our internal control over financial reporting that occurred during the three months ended ~~March~~June 3130, 2025 that have materially affected, or are reasonably likely to materially affect, our internal control over financial reporting.

3233

Part II - Other Information

Item 1. Legal Proceedings

~~From~~We ~~time~~are tocurrently ~~time~~engaged in arbitration proceedings with Beam Therapeutics, Inc. (“Beam”) regarding the parties’ collaboration and license agreement (the “Agreement”). A dispute arose between the parties following our March 18, 2025 announcement that we ~~may~~are ~~become~~developing ~~involved~~a inPrime ~~litigation~~Editing-based ortreatment ~~other~~for ~~legal~~alpha-1 ~~proceedings~~antitrypsin deficiency (“AATD”). ~~While~~On April 16, 2025, Beam filed an arbitration demand with the ~~outcome~~American Arbitration Association (“AAA”), alleging that we have breached the Agreement by developing a product for the treatment of ~~any~~AATD ~~such~~and ~~proceedings~~by ~~cannot~~allegedly benot ~~predicted~~complying with ~~certainty~~certain obligations to transfer technical information to Beam pursuant to the Agreement. Beam also makes related claims for trade secret misappropriation and various business torts based on similar allegations, asincluding allegations made on information and belief. Beam seeks both declaratory, injunctive, and monetary relief, but has not yet quantified the amount of ~~March 31~~damages it seeks. On April 18, 2025, we ~~were~~filed ~~not~~an aarbitration ~~party~~demand towith ~~any~~the ~~litigation~~AAA orseeking ~~legal~~a ~~proceedings~~declaration that our AATD program is within our “Field” as defined by the Agreement. The arbitrations have been consolidated, and the consolidated proceeding remains in its early stages. If the ~~opinion~~final resolution of ~~our~~the ~~management,~~matter ~~are~~is ~~probable~~adverse to ~~have~~us, athe ~~material~~arbitration ~~adverse~~panel ~~effect~~may provide Beam with relief including, among other things, monetary damages and/or an order that we cease work on our ~~business.~~AATD program and transfer such program to Beam.

Item 1A. Risk Factors

Investing in our common stock involves a high degree of risk. For a detailed discussion of the risks and uncertainties related to our business, please refer to the section titled “Risk Factors” in our Annual Report on Form 10-K for the year ended December 31, 2024. There have been no material changes from the risk factors set forth in our Annual Report on Form 10-K for the year ended December 31, 2024, except as set forth below.

~~Because~~Healthcare ~~our existing cash, cash equivalents,~~ and ~~investments~~other ~~will~~reform ~~not~~legislation bemay ~~sufficient~~increase tothe ~~fund~~difficulty ~~our~~and ~~operations,~~cost asfor ~~currently~~us ~~planned,~~and ~~for~~any ~~more~~collaborators ~~than~~we ~~one~~may ~~year~~have ~~beyond~~to ~~the~~obtain ~~filing~~marketing ~~date~~approval of ~~this~~and ~~Quarterly~~commercialize ~~Report~~any onproduct ~~Form~~candidates ~~10-Q,~~ we ~~have~~may ~~determined~~develop ~~that~~and ~~there~~affect isthe ~~substantial~~prices ~~doubt~~we, ~~regarding~~or ~~our~~they, ~~ability~~may ~~to continue as a going concern~~obtain.

~~Based~~In onthe ~~our~~United ~~existing~~States ~~cash, cash equivalents, short-term investments,~~ and ~~related~~some ~~party~~foreign ~~short-term investments as of March 31~~jurisdictions, ~~2025,~~there ~~our~~have ~~current~~been and ~~forecasted level of operations and our forecasted cash flows, our ability to~~ continue ~~as a going concern is dependent upon our ability~~ to ~~obtain~~be ~~the~~ongoing ~~necessary~~efforts ~~financing~~ to ~~meet~~implement ~~our~~legislative ~~obligations~~ and ~~repay~~regulatory ~~our~~changes ~~liabilities~~regarding ~~arising~~the ~~from~~healthcare ~~normal~~system. ~~business~~Such ~~operations~~changes ~~when~~could ~~they~~prevent ~~come~~or ~~due.~~delay Wemarketing ~~plan~~approval toof ~~provide~~any ~~for~~product ~~our~~candidates ~~capital~~that ~~requirements~~we ~~through~~may ~~financing or other transactions~~develop, ~~and~~restrict ~~selling~~or ~~shares~~regulate ofpost-approval ~~our~~activities ~~common~~and ~~stock~~affect ~~under~~ our ~~“at~~ability ~~the~~to ~~market~~profitably ~~offering”~~sell ~~program.~~any ~~There~~product ~~can~~candidates befor nowhich ~~assurance that~~ we ~~will~~obtain bemarketing ~~able~~approval. toAlthough ~~raise~~we ~~additional~~cannot ~~capital~~predict towhat ~~fund~~healthcare ~~operations with terms acceptable to us,~~ or atother ~~all.~~reform ~~Because~~efforts ~~our existing cash, cash equivalents, and investments~~ will ~~not~~ be ~~sufficient~~successful, tosuch ~~fund~~efforts ~~our~~may ~~operations~~result ~~for~~in more ~~than~~rigorous ~~one~~coverage ~~year~~criteria, ~~from~~in ~~the~~additional ~~date~~downward ofpressure ~~issuance~~on of the ~~consolidated~~price ~~financial~~that ~~statements~~we, ~~appearing~~or inour ~~this~~future ~~Quarterly~~collaborators, ~~Report~~may onreceive ~~Form~~for ~~10-Q,~~any weapproved ~~have~~products ~~determined~~or ~~that~~in ~~there~~other isconsequences ~~substantial~~that ~~doubt~~may ~~regarding~~adversely affect our ability to ~~continue~~achieve asor amaintain ~~going concern~~profitability.

~~The~~Within ~~substantial~~the ~~doubt~~United ~~about~~States, ~~our~~the ~~ability~~federal togovernment ~~continue~~and individual states have aggressively pursued healthcare reform, as aevidenced ~~going~~by ~~concern~~the ~~may~~passing ~~adversely~~of ~~affect~~the ~~our~~Affordable ~~stock~~Care ~~price~~Act, as amended by the Health Care and ~~our~~Education ~~ability~~Reconciliation Act of 2010 (the “ACA”), and the ongoing efforts to ~~raise~~modify ~~capital~~or repeal that legislation. IfThe weACA ~~are~~substantially ~~unable~~changed tothe ~~obtain~~way ~~additional~~healthcare ~~capital,~~is wefinanced ~~may~~by ~~not~~both begovernmental ~~able~~and toprivate ~~continue~~insurers ~~our~~and ~~operations~~contains ona ~~the~~number ~~scope~~of provisions that affect coverage and reimbursement of drug products and/or ~~scale~~that could potentially reduce the demand for pharmaceutical products such as ~~currently~~increasing ~~conducted~~drug rebates under state Medicaid programs for brand name prescription drugs and extending those rebates to Medicaid managed care and assessing a fee on manufacturers and importers of brand name prescription drugs reimbursed under certain government programs, including Medicare and Medicaid. Other aspects of healthcare reform, such as expanded government enforcement authority and heightened standards that could ~~have~~increase acompliance-related ~~material~~costs, ~~adverse~~could ~~effect~~also onaffect our business,. ~~results~~Modifications ofhave ~~operations~~been implemented under the former Trump administration and ~~financial~~additional ~~condition~~modifications or repeal may occur.

~~Disruptions~~The atcontinuing ~~the~~efforts ~~FDA~~of ~~and~~the ~~other~~ government ~~agencies caused by reduction in staffing~~, ~~funding~~insurance ~~shortages or global health concerns could hinder their ability to hire~~companies, ~~retain~~managed orcare ~~deploy~~organizations ~~key leadership~~ and other ~~personnel,~~payers orof ~~otherwise~~healthcare ~~prevent~~services ~~new~~to orcontain ~~modified products from being developed, approved,~~ or ~~commercialized~~reduce incosts aof ~~timely~~healthcare ~~manner~~may oradversely ataffect: ~~all, which could negatively impact our business.~~

~~Currently, federal agencies in~~ •the ~~U.S.~~demand ~~are~~for ~~operating~~any ~~under a continuing resolution that is set to expire on September 30, 2025. Without appropriation~~ of ~~additional funding to federal agencies,~~ our ~~business operations related to our~~ product ~~development activities for the U.S. market could be impacted. Inadequate funding for the FDA~~candidates, ~~the~~if ~~SEC~~approved; and other government agencies, including from government shut downs, or other disruptions to these agencies’ operations, could hinder their ability to hire and retain key leadership and other personnel, prevent new products and services from being developed or commercialized in a timely manner or otherwise prevent those agencies from performing normal business functions on which the operation of our business may rely, which could negatively impact our business. The Trump administration has issued executive orders seeking to greatly reduce the size of the federal workforce, including through layoffs and severance packages offered to employees of federal agencies within the executive branch and independent agencies, including the FDA. Any such reduction in personnel may result in longer review times by the FDA and other agencies.

The ability of the FDA to review and approve new products can be affected by a variety of factors, including government budget and funding levels, statutory, regulatory, and policy changes, the FDA’s ability to hire and retain key personnel and accept the payment of user fees, and other events that may otherwise affect the FDA’s ability to perform routine functions. Average review times at the agency have fluctuated in recent years as a result. In

addition, government funding of other government agencies that fund research and development activities is subject to the political process, which is inherently fluid and unpredictable. Disruptions and personnel turnover, as a result of leadership changes, staff reductions or otherwise, at the FDA and other agencies may also slow the time necessary for biologics or modifications to approved biologics to be reviewed and/or approved by necessary government agencies, which would adversely affect our business. Changes and cuts in FDA staffing also could result in delays in the FDA’s responsiveness or in its ability to review IND submissions or applications, issue regulations or guidance, or implement or enforce regulatory requirements in a timely fashion or at all.

Over the last several years, the U.S. government has shut down several times and certain regulatory agencies, such as the FDA, have had to furlough critical employees and stop critical activities. If a prolonged government shutdown occurs, or if staffing changes prevent the FDA or other regulatory authorities from conducting their regular inspections, reviews, or other regulatory activities, including formal and informal interactions with product developers, it could significantly impact the ability of the FDA or other regulatory authorities to timely review and process our future regulatory submissions, which could have a material adverse effect on our business.

Unfavorable macroeconomic conditions or market volatility resulting from geopolitical developments or national or global economic conditions, including those affecting the financial services industry, could adversely affect our business, financial condition or results of operations.

Adverse macroeconomic conditions or market volatility resulting from national or global economic developments, political unrest, high inflation, rising interest rates, new or increase international tariffs and retaliatory tariffs, changes in international trade relationships and military conflicts, such as the ongoing conflict between Russia and Ukraine, the potential for significant changes in U.S. policies or regulatory environment, or other factors, could materially and adversely affect our business operations. Sanctions imposed by the U.S. and other countries in response to such conflicts may also continue to adversely impact the financial markets and the global economy, and any economic countermeasures by the affected countries or others could exacerbate market and economic instability. Tariffs levied by the U.S. and other countries also may adversely affect financial markets and the global economy. For example, on April 2, 2025, the U.S. imposed substantial tariffs on most countries throughout the world. The new U.S. administration has threatened to continue to broadly impose tariffs, which could lead to corresponding punitive actions by the countries with which the U.S. trades.

Additionally, changes to policy implemented by the U.S. Congress, the Trump administration or any new administration have impacted and may in the future impact, among other things, the U.S. and global economy, international trade relations, unemployment, immigration, healthcare, taxation, the U.S. regulatory environment, inflation and other areas. There can be no assurance that further deterioration in credit and financial markets and confidence in economic conditions will not occur. For instance, actual events involving limited liquidity, defaults, non-performance or other adverse developments that affect financial institutions, transactional counterparties or other companies in the financial services industry or the financial services industry generally, or concerns or rumors about any events of these kinds or other similar risks, have in the past and may in the future lead to market-wide liquidity problems. Investor concerns regarding the U.S. or international financial systems could result in less favorable commercial financing terms, including higher interest rates or costs and tighter financial and operating covenants, or systemic limitations on access to credit and liquidity sources, thereby making it more difficult for us to acquire financing on acceptable terms or at all. In addition, any further deterioration in the macroeconomic economy or financial services industry could lead to losses or defaults by our suppliers, which in turn, could have a material adverse effect on our current and/or planned business operations and our current or projected results of operations and financial condition. For example, there has been proposed U.S. legislation that may restrict the ability of U.S. biopharmaceutical companies to purchase services or products from, or otherwise collaborate with, certain Chinese biotechnology companies of concern without losing the ability to contract with, or otherwise receive funding from, the U.S. government. We continue to assess the legislation as it develops to determine whether it could have an effect on our contractual relationships. Also, current inflationary trends in the global economy may impact salaries and wages, costs of goods and transportation expenses, among other things, and recent and potential future disruptions in access to bank deposits or lending commitments due to bank failures may create market and economic instability.

A•the ~~severe~~ability orto ~~prolonged~~set ~~economic downturn or additional global financial crises could result in~~ a ~~variety~~price ofthat ~~risks~~we tobelieve ~~our~~is ~~business,~~fair ~~including weakened demand~~ for any ~~product~~of ~~candidates we develop or~~ our ~~ability~~product ~~to raise additional capital when needed on acceptable terms~~candidates, if atapproved; ~~all.~~

~~Further,~~•our ~~U.S.~~ability government appropriations have been affected by larger U.S. government budgetary issues and related legislation. In addition, in the past, U.S. debt ceiling and budget deficit concerns have increased the possibility of additional credit-rating downgrades and economic slowdowns, or a recession in the U.S. Although U.S. lawmakers passed legislation to ~~raise~~generate ~~the~~revenues federal debt ceiling on multiple occasions, ratings agencies have lowered or threatened to lower the long-term sovereign credit rating on the U.S. The impact of this or any further downgrades to the U.S. government’s sovereign credit rating or its perceived creditworthiness could adversely affect the U.S. and ~~global~~achieve financial markets and economic conditions. As a result, government spending levels are difficult to predict beyond the near term due to numerous factors, including the external threat environment, future government priorities and the state of government finances. Significant changes in government spending or ~~changes~~maintain inprofitability; ~~U.S. government priorities, policies and requirements could have a material adverse effect on our results of operations, financial condition or liquidity.~~

~~Any of~~ •the ~~foregoing~~level ~~could~~of ~~harm~~taxes ~~our~~that ~~business and~~ we ~~cannot~~are ~~anticipate~~required ~~all~~to ofpay; ~~the ways in which the current economic climate~~ and ~~financial market conditions could adversely impact our business.~~

•the availability of capital.

Legislative and regulatory proposals have been made to expand post-approval requirements and restrict sales and promotional activities for pharmaceutical and biologic products. We cannot be sure whether additional legislative changes will be enacted, or whether FDA regulations, guidance or interpretations will be changed, or what the impact of such changes on the marketing approvals of our product candidates, if any, may be. In addition, increased scrutiny by Congress of the FDA’s approval process may significantly delay or prevent marketing approval, as well as subject us to more stringent product labeling and post-marketing testing and other requirements.

Moreover, increasing efforts by governmental and third-party payors in the United States and abroad to cap or reduce healthcare costs may cause such organizations to limit both coverage and the level of reimbursement for newly approved products and, as a result, they may not cover or provide adequate payment for our product candidates. There has been increasing legislative and enforcement interest in the United States with respect to specialty drug pricing practices. Specifically, there have been several recent U.S. Congressional inquiries and proposed and enacted federal and state legislation designed to, among other things, bring more transparency to drug pricing, reduce the cost of prescription drugs under Medicare, review the relationship between pricing and manufacturer patient programs, and reform government program reimbursement methodologies for drugs. For example, the Inflation Reduction Act of 2022 (the “IRA”) that was signed into law in August 2022, contains several provisions that are intended to limit prices of pharmaceutical and biologic products, including creating a $2,000 out-of-pocket cap for Medicare Part D beneficiaries, imposing new manufacturer financial liability on all drugs in Medicare Part D, allowing the U.S. government to negotiate Medicare Part B and Part D pricing for certain high-cost pharmaceutical and biologic drugs without generic or biosimilar competition, requiring companies to pay rebates to Medicare for drug prices that increase faster than inflation, and delaying until January 1, 2032 the implementation of the U.S. Department of Health and Human Services (“HHS”) rebate rule that would have limited the fees that pharmacy benefit managers can charge. Further, under the IRA, orphan drugs were previously exempted from the Medicare drug price negotiation program; however, this exemption was restricted to drugs with only one orphan designation and for which the only approved indication is for that disease or condition. If a product received multiple orphan designations or had multiple approved indications, it would not qualify for the orphan drug exemption. Under the One Big Beautiful Bill Act of 2025, this restriction was eliminated; and effective for the 2028 initial price applicability year, all orphan drugs, regardless of the number of orphan designations or indications, are exempt from the Medicare drug price negotiation program. The effects of the IRA on our business and the healthcare industry in general is not yet known.

On April 15, 2025, the Trump Administration published Executive Order 14273, “Lowering Drug Prices by Once Again Putting Americans First,” which generally directs the HHS to take measures to reduce drug prices, including eliminating the so-called “pill penalty” under the IRA that creates a distinction between small molecule and large molecule products for purposes of determining when a drug may be eligible for drug price negotiation. On May 12, 2025, the Trump Administration published Executive Order 14297, “Delivering Most-Favored-Nation Prescription Drug Pricing to American Patients” which generally, among other things, directs certain executive officials to establish and communicate most-favored-nation price targets to pharmaceutical manufacturers to bring prices for American patients in line with comparably developed nations. Further, the Executive Order directs the federal government to support regulatory paths to allow direct-to-patient sales for companies that meet these targets. It also states that the Administration will take additional aggressive action (for example, examining whether marketing approvals should be modified or rescinded or opening the door for individual drug importation waivers) should manufacturers fail to offer American consumers the most-favored-nation lowest price. It also directs the Secretary of Commerce and the U.S. Trade Representative to “take all necessary and appropriate action to ensure foreign countries are not engaged in any act, policy, or practice that may be unreasonable or discriminatory or that may impair United States national security . . . including by suppressing the price of pharmaceutical products below fair market value in foreign countries.” Notably, a similar “Most Favored Nation” pricing rule enacted under the first

Trump Administration was subject to an injunction resulting from judicial challenges to the rule, which was formally rescinded by the former Biden Administration in August 2021.

In addition, at the state level, legislatures have increasingly passed legislation and implemented regulations similar to those under consideration at the federal level, as well as laws designed to control pharmaceutical and biotherapeutic product pricing, including restrictions on pricing or reimbursement at the state government level, limitations on discounts to patients, marketing cost disclosure and transparency measures, restrictions or other limitations on patient assistance, and, in some cases, policies to encourage importation from other countries (subject to federal approval) and bulk purchasing. Certain states are also pursuing cost containment efforts through Prescription Drug Affordability Boards and similar entities.

We expect that the healthcare reform measures that have been adopted and may be adopted in the future may result in more rigorous coverage criteria and in additional downward pressure on the price that we receive for any approved product and could seriously harm our future revenues. We cannot be sure whether additional legislative changes will be enacted, or whether FDA regulations, guidance or interpretations will be changed, or what the impact of such changes on the marketing approvals of our products and future product candidates, if any, may be. In addition, increased scrutiny by Congress of the FDA’s approval process may significantly delay or prevent marketing approval, as well as subject us to more stringent product labeling and post-marketing testing and other requirements. Any reduction in reimbursement from Medicare or other government programs may result in a similar reduction in payments from private payors. The implementation of cost containment measures or other healthcare reforms may prevent us from being able to generate revenue, attain profitability or commercialize our products.

The U.S. Congress, the Trump administration, or any new administration may make substantial changes to fiscal, tax, and other federal policies that may adversely affect our business.

Since the start of the Trump Administration in 2025, U.S. policy changes have been implemented at a rapid pace and additional changes are likely. For example, the U.S. government has adopted new approaches to trade policy and in some cases may renegotiate, or potentially terminate, certain existing bilateral or multi-lateral trade agreements. The U.S. government has also imposed substantial tariffs on most countries throughout the world and has further threatened to continue to broadly impose tariffs, which could lead to corresponding punitive actions by the countries with which the U.S. trades. While certain tariffs have subsequently been suspended, modified or temporarily reduced, we cannot predict the results of the U.S. government’s trade negotiations or the outcome of ongoing legal challenges to specific tariff policies. Changes to U.S. policy implemented by the U.S. Congress, the Trump administration or any new administration have impacted and may in the future impact, among other things, the U.S. and global economy, international trade relations, unemployment, immigration, healthcare, taxation, the U.S. regulatory environment, inflation and other areas. Although we cannot predict the impact, if any, of these changes to our business, they could adversely affect our business. Until we know what policy changes are made, whether those policy changes are challenged and subsequently upheld by the court system and how those changes impact our business and the business of our competitors over the long term, we will not know if, overall, we will benefit from them or be negatively affected by them.

Changes in tax laws or in their implementation or interpretation may adversely affect our business and financial condition.

The rules dealing with U.S. federal, state and local income taxation are constantly under review by persons involved in the legislative process and by the Internal Revenue Service, the U.S. Treasury Department and non-U.S. taxing authorities. For example, the One Big Beautiful Bill Act (the “OBBBA”) was signed into law on July 4, 2025 and made significant changes to U.S. federal tax law. Changes to tax laws (which changes may have retroactive application) could adversely affect our business and our financial condition. For example, under Section 174 of the Internal Revenue Code of 1986, as amended, in taxable years beginning after December 31, 2021, expenses that are incurred for research and development performed outside the U.S. will be capitalized and amortized, which may have an adverse effect on our cash flow. The OBBBA provides that for taxable years beginning after December 31, 2024, expenses that are incurred for research and development performed in the U.S. may, at the taxpayer’s election, be immediately deducted or capitalized and amortized. In addition, the OBBBA provides that for taxable years beginning after December 31, 2021 and before January 1, 2025, certain eligible taxpayers generally may elect to

retroactively deduct expenses for research and development performed in the U.S. in such taxable years by filing amended tax returns for such taxable years, and all other taxpayers that are not eligible to make such an election and that amortized expenses for research and development performed in the U.S. in such taxable years generally may elect to accelerate and deduct the remaining unamortized amounts of such research and development expenses (i) in the first taxable year beginning after December 31, 2024, or (ii) ratably over the two-taxable year period beginning with the first taxable year beginning after December 31, 2024. In recent years, many such changes have been made and changes are likely to continue to occur in the future. We cannot predict whether, when, in what form or with what effective dates, tax laws, regulations and rulings may be enacted, promulgated or decided or whether they could increase our tax liability or require changes in the manner in which we operate in order to minimize increases in our tax liability.

Our future success depends on our ability to retain our key employees and to attract, retain and motivate qualified personnel.

We are highly dependent on our key executives and other principal members of our management, scientific and clinical team. Although we have entered into employment agreements and/or offer letters with our executive officers, they are engaged “at will,” meaning we or they may terminate the relationship at any time. We do not maintain “key person” insurance for any of our executives or other employees. The loss of the services of any of these persons could impede the achievement of our research, development and commercialization objectives.

Recruiting and retaining qualified scientific, clinical, manufacturing and sales and marketing personnel will also be critical to our success. In addition, our company-building efforts and establishment of a company culture will also be important to developing an innovative company in a high-evolving area. We may not be able to succeed in these efforts to build Prime Medicine as an attractive and exciting place to build a career or to attract and retain these types of personnel on acceptable terms given the competition among numerous pharmaceutical and biotechnology companies for similar personnel. We also experience competition for the hiring of scientific and clinical personnel from universities and research institutions. In addition, we rely on consultants and advisors, including scientific and clinical advisors, to assist us in formulating our research and development and commercialization strategy. Our consultants and advisors, may be employed by employers other than us and may have commitments under consulting or advisory contracts with other entities that may limit their availability to us. The inability to recruit, or loss of services of, certain executives, key employees, consultants or advisors, may impede the progress of our research, development and commercialization objectives and have a material adverse effect on our business, financial condition, results of operations and prospects.

To motivate and retain qualified employees, executive officers and directors who we believe best represent our Company values and can make meaningful contributions towards achieving our purpose of delivering a new class of differentiated one-time curative genetic therapies to address the widest spectrum of diseases by deploying our Prime Editing technology, in addition to salary and cash incentives, as applicable, we have provided stock options that vest over time. The value to employees and directors of stock options that vest over time may be significantly affected by movements in our stock price that are beyond our control and may at any time be insufficient to counteract more lucrative offers from other companies. To the extent our stock price declines, our ability to incentivize, retain or attract qualified talent could be negatively impacted. For example, in recent years, the stock market in general, and the market for pharmaceutical and biotechnology companies in particular, has experienced extreme price and volume fluctuations, often unrelated or disproportionate to changes in the operating performance of the affected companies. As a biotechnology company, the market price of our common stock has historically been volatile, reflecting the risks and uncertainties inherent in the development of product candidates. Since 2024, the market price of our common stock has experienced material fluctuations and declined from a high of approximately $9.39 on February 27, 2024 to a low of approximately $1.15 on April 8, 2025. As a result, certain of our employees and directors now hold options with exercise prices meaningfully above the recent trading range of our common stock (often referred to as “underwater” or “out-of-the-money”), rendering the options a less effective means of incentivizing and retaining such holders.

We also recently announced a strategic restructuring, including the deprioritization of our CGD programs, as well as a cost and workforce reduction to focus on our liver franchise and programs funded through external partnerships, placing additional pressure on the retention of qualified talent. Although we continue to believe that stock options

are an important component of the Company’s compensation program, underwater options may be perceived by their holders as having little or no incentive or retention value due to the disparity between the exercise prices and the current stock price. To provide added incentives to retain and motivate key contributors and to improve morale among our employees and directors to ensure alignment and motivation to execute on the Company’s reprioritized strategy, our stockholders approved a one-time repricing of certain outstanding stock options that have been granted under our 2019 Stock Option and Grant Plan and/or the 2022 Stock Option and Incentive Plan at the special meeting of stockholders held on August 1, 2025. Despite this, we may have difficulty retaining key personnel, which could adversely affect our business and further development of our product candidates.

If conflicts arise between us and our collaborators or strategic partners, these parties may act in a manner adverse to us and could limit our ability to implement our strategies or we could lose license rights that are important to our business.

We are and expect to continue to be reliant upon certain patent rights and proprietary technology we have licensed from third parties that may be important or necessary to the development of our Prime Editing technology and product candidates. If conflicts arise between our corporate or academic collaborators or strategic partners and us, the other party may act in a manner adverse to us and could limit our ability to implement our strategies or we could lose license rights that are important to our business. For instance, we have entered into license and collaboration agreements with Beam and Broad Institute related to the research, development, delivery, manufacturing, and commercialization of Prime Editing technology and certain product candidates we may develop.

The agreements under which we currently license intellectual property rights from Beam and Broad Institute are complex, and certain provisions in such agreements may be susceptible to multiple interpretations. The resolution of any contract interpretation disagreement that may arise under our existing license agreements or future license agreements into which we may enter could narrow what we believe to be the scope of our rights to the relevant intellectual property or technology or broaden what we believe to be the scope of the licensor’s rights to our intellectual property and technology, or increase what we believe to be our financial or other obligations under the relevant agreement, any of which could have a material adverse effect on our business, financial condition, results of operations and prospects. For example, we have exclusively licensed and sublicensed certain of our owned and licensed intellectual property rights to Beam pursuant to a license and collaboration agreement, or the Beam Collaboration Agreement, in certain fields. The parties have presented differing contractual interpretations, the resolution of which could expand the field of exclusivity or other rights that we believe were granted to Beam and therefore, narrow what we believe to be our field of exclusivity or rights with respect to such licensed intellectual property rights.

For example, we are currently engaged in arbitration proceedings with Beam regarding the Beam Collaboration Agreement. A dispute arose between the parties following our March 18, 2025 announcement that we are developing a Prime Editing-based treatment for AATD. On April 16, 2025, Beam filed an arbitration demand with the American Arbitration Association, or the AAA, alleging that we have breached the Beam Collaboration Agreement by developing a product for the treatment of AATD and by allegedly not complying with certain obligations to transfer technical information to Beam pursuant to the Beam Collaboration Agreement. On April 18, 2025, we filed an arbitration demand with the AAA seeking a declaration that our AATD program is within our “Field” as defined by the Beam Collaboration Agreement. The arbitrations have been consolidated, and the consolidated proceeding remains in its early stages. If the final resolution of the matter is adverse to us, the arbitration panel may provide Beam with relief including, among other things, monetary damages and/or an order that we cease work on our AATD program and transfer such program to Beam. Such a relief could have a material adverse effect on our competitive position, business, financial condition, results of operations and growth prospects.

Our strategic restructuring and the associated workforce reduction announced in May 2025 may not result in anticipated cost savings, could result in total costs and expenses that are greater than expected and could disrupt our business.

In May 2025, we announced a strategic restructuring, including the deprioritization of our CGD programs, to focus on our liver franchise and programs funded through external pipelines. The strategic restructuring includes cost reduction measures and reduction of our organizational headcount by approximately 25%, which are designed to

significantly decrease our operating expenses and cash burn, reducing anticipated cash needs by almost half through 2027. The estimates of the charges and cash expenditures that we expect to incur in connection with the strategic restructuring and related workforce reduction, and the timing thereof, are subject to a number of assumptions, and we may incur costs that are greater than we currently expect. We may not realize, in full or in part, the anticipated benefits, savings and improvements in our operating structure from our new strategic efforts due to unforeseen difficulties, delays or unexpected costs. If we are unable to realize the expected operational efficiencies and cost savings from the strategic restructuring, our results of operation and financial condition would be adversely affected. We also cannot guarantee that we will not have to undertake additional workforce reductions or related activities in the future. Such cost reduction efforts may in the future adversely affect our ability to attract and retain employees, and may adversely affect our culture and impact our ability to effectively pursue our business strategy. Furthermore, our strategic restructuring may be disruptive to our operations. For example, our workforce reductions could yield unanticipated consequences, such as attrition beyond planned staff reductions, increased difficulties in our day-to-day operations and reduced employee morale. If employees who were not affected by the reduction in force seek alternate employment, this could result in us seeking contract support which may result in unplanned additional expense or harm our productivity. Our workforce reductions could also harm our ability to attract and retain qualified management, scientific, and clinical personnel who are critical to our business. Any failure to attract or retain qualified personnel could prevent us from successfully developing our product candidates in the future.

Item 2. Unregistered Sales of Equity Securities and Use of Proceeds

Set forth below is information regarding shares of equity securities sold, and options granted, by us during the three months ended ~~March~~June 3130, 2025 that were not registered under the Securities Act.

Recent Sales of Unregistered Equity Securities

None.

Issuer Purchases of Equity Securities

None.

Item 3. Defaults Upon Senior Securities

None.

Item 4. Mine Safety Disclosures

Not Applicable.

Item 5. Other Information

Rule 10b5-1 Trading Arrangements

From time to time, our officers (as defined in Rule 16a–1(f)) and directors may enter into Rule 10b5-1 or non-Rule 10b5-1 trading arrangements (as each such term is defined in Item 408 of Regulation S-K). During the three months ended ~~March~~June 3130, 2025, none of the Company’s directors or officers adopted, modified or terminated a plan or other arrangement intended to satisfy the affirmative defense conditions of Rule 10b5-1(c) or any non-Rule 10b5-1 trading arrangements (as defined in Item 408(c) of Regulation S-K).

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Item 6. Exhibits

(a)Exhibits.

Exhibit number						Exhibit table
3.1						Third Amended and Restated Certificate of Incorporation of Prime Medicine, Inc. (incorporated by reference to Exhibit 3.1 to the Registrant’s Current Report on Form 8-K, filed with the SEC on October 24, 2022).
3.2						Certificate of Amendment to Third Amended and Restated Certificate of Incorporation of Prime Medicine, Inc. (incorporated by reference to Exhibit 3.1 to the Registrant’s Current Report on Form 8-K, filed with the SEC on June 12, 2024).
3.3						Second Amended and Restated Bylaws of Prime Medicine, Inc. (incorporated by reference to Exhibit 3.1 to the Registrant’s Current Report on Form 8-K, filed with the SEC on May 21, 2024).
31.1*						Certification of Principal Executive Officer Pursuant to Rules 13a-14(a) and 15d-14(a) under the Securities Exchange Act of 1934, as Adopted Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002.
10.1*#						Second Amended and Restated Non-Employee Director Compensation Policy
31.2*						Certification of Principal Financial Officer Pursuant to Rules 13a-14(a) and 15d-14(a) under the Securities Exchange Act of 1934, as Adopted Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002.
10.2*#						Separation Agreement, dated May 18, 2025, between the Registrant and Keith Gottesdiener
32.1**						Certification of Principal Executive Officer Pursuant to 18 U.S.C. Section 1350, as Adopted Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002.
10.3*#†						Consulting Agreement, dated May 18, 2025, between the Registrant and KMG Strategic Consulting LLC
32.2**						Certification of Principal Financial Officer Pursuant to 18 U.S.C. Section 1350, as Adopted Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002.
10.4*#						Amended and Restated Employment Agreement, effective May 19, 2025, between the Registrant and Allan Reine
10.5*#+						Separation Agreement, dated July 17, 2025, between the Registrant and Jeremy Duffield
10.6*#						Consulting Agreement, dated July 15, 2025, between the Registrant and Jeremy Duffield
31.1*						Certification of Principal Executive Officer and Principal Financial Officer Pursuant to Rules 13a-14(a) and 15d-14(a) under the Securities Exchange Act of 1934, as Adopted Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002.
32.1**						Certification of Principal Executive Officer and Principal Financial Officer Pursuant to 18 U.S.C. Section 1350, as Adopted Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002.
101.INS*						Inline XBRL Instance Document
101.SCH*						Inline XBRL Taxonomy Extension Schema Document
101.CAL*						Inline XBRL Taxonomy Extension Calculation Linkbase Document
101.DEF*						Inline XBRL Taxonomy Extension Definition Linkbase Document
101.LAB*						Inline XBRL Taxonomy Extension Label Linkbase Document
101.PRE*						Inline XBRL Taxonomy Extension Presentation Linkbase Document
104*						Cover Page Interactive Data File (embedded within the Inline XBRL document)

__________________

*Filed herewith.

** The ~~certifications~~certification furnished in Exhibit 32.1 ~~and Exhibit 32.2~~ hereto ~~are~~is deemed to accompany this Quarterly Report on Form 10-Q and will not be deemed “filed” for purposes of Section 18 of the Securities Exchange Act of 1934, as amended. Such ~~certifications~~certification will not be deemed to be incorporated by reference into any filings under the Securities Act of 1933, as amended, or the Securities Exchange Act of 1934, as amended, except to the extent that the Registrant specifically incorporates it by reference.

# Indicates a management contract or any compensatory plan, contract or arrangement.

† Portions of this exhibit (indicated by asterisks) have been omitted in accordance with Item 601(b)(10) of Regulation S-K.

+ Annexes, schedules and/or exhibits have been omitted pursuant to Item 601(a)(5) of Regulation S-K. The Registrant agrees to furnish supplementally a copy of any omitted attachment to the SEC on a confidential basis upon request.

(b)Financial Statement Schedules.

None.

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Signatures

Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized.

Prime Medicine, Inc.

Date:

By:

/s/ Keith Gottesdiener

By:

/s/ Allan Reine

Date: May 08, 2025

Keith Gottesdiener

Date: August 7, 2025

Allan Reine

President and Chief Executive Officer(Principal Executive Officer)

Chief Executive Officer(Principal Executive Officer and Principal Financial Officer)

Date: May 08, 2025

By:

/s/ Allan Reine

Allan Reine

Chief Financial Officer(Principal Financial Officer)

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Filing Compare · Prime Medicine, Inc.