EXHIBIT 21.1

ASP Isotopes Inc.

Subsidiaries of the Registrant

The following is a list of subsidiaries of ASP Isotopes Inc. as of March 31, 2026:


Subsidiaries*		Place of Incorporation
ASP Isotopes Guernsey Limited (formerly, PDS-Photonica Holdings (Guernsey) Limited)		Guernsey
ASP Isotopes South Africa (Proprietary) Limited (formerly, PDS Photonica Holdings South Africa (PTY) Limited)		South Africa
Enlightened Isotopes (Pty) Ltd**		South Africa
ASPI South Africa Asset Finance		South Africa
Enriched Energy LLC		Delaware, U.S.
ASP Isotopes UK Ltd		England & Wales
ASP Isotopes Services (UK) Limited		England & Wales
Enlightened Isotopes (Pty) Ltd		South Africa
ASP Isotopes ehf		Iceland
Quantum Leap Energy LLC		Delaware, U.S.
Quantum Leap Energy Limited		England & Wales
Quantum Leap Energy (Pty) Ltd		South Africa
QLE TP Funding SPE LLC		Delaware, U.S.
K2025267858 (South Africa) (Pty) Ltd		South Africa
Global Radiopharmacies LLC		Delaware, U.S.
East Coast Nuclear Pharmacy LLC		Florida, U.S.
NuMed Diagnostics, LLC**		South Carolina, U.S.
PET Labs Pharmaceuticals (Pty) Ltd**		South Africa
PET Labs Global Nuclear Medicine SEZC		Cayman Islands
Renergen Limited		South Africa
Tetra4 Proprietary Limited**		South Africa
Cryovation Proprietary Limited		South Africa
Renergen US Inc.		Delaware, U.S.
Skyline Builders Group Holding Limited**		Hong Kong


	*	Please note that this list includes all subsidiaries of ASP Isotopes Inc. without regard to whether they would constitute a "significant subsidiary" pursuant to Item 601(b)(21)(ii) of Regulation S-K.
	*	Not a wholly-owned direct or indirect subsidiary of ASP Isotopes Inc.

ASP Isotopes Inc. 10-K